| 刘文励,黄伟,何美冬,孙汉英,肖侃艳.复方活血汤对辐射损伤小鼠骨髓基质细胞粘附功能的影响[J].,1997,(6):354-355 |
| 复方活血汤对辐射损伤小鼠骨髓基质细胞粘附功能的影响 |
| Effects of Composite Blood-Activating Decoction on Adhesive Function of Bone Marrow Stroma Cells in Radiation Injured Mice |
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| DOI： |
| 中文关键词: 放射损伤 基质细胞 骨髓 粘附能力 活血化瘀中药 |
| 英文关键词:radioactive injurey bone marrow stroma cell adhesion function bloodactivating and stasis removing Chinese herbal drugs |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 目的：研究常用活血化瘀中药对急性放射损伤小鼠骨髓基质细胞粘附功能的影响。方法：小鼠接受８ＧＹ６０Ｃｏγ射线照射后立即腹腔注射１００％复方活血汤液０．２ｍｌ，每天２次，连续６天，第７天取股骨骨髓制备有核细胞（ｂｏｎｅｍａｒｒｏｗｃｅｌｌ，ＢＭＣ）悬液，按长期骨髓细胞培养技术，第１２天计数成纤维细胞集落形成单位（ｆｉｂｒｏｂｌａｓｔｃｏｌｏｎｙｆｏｒｍｉｎｇｕｎｉｔ，ＣＦＵ－Ｆ），第２８天观察培养的基质细胞层对正常小鼠ＢＭＣ的粘附能力。结果：正常小鼠骨髓培养的基质细胞层粘附正常小鼠ＢＭＣ的能力为６７．８±１７．２％，复方活血汤组为５５．８±１０．５％，差异无显著意义（Ｐ＞０．０５）；对照组为４７．７±１３．６％，显著低于正常组（Ｐ＜０．０２）。结论：常用活血化瘀中药能增强急性放射损伤小鼠骨髓基质细胞粘附功能。 |
| 英文摘要: |
| To evaluate the effect of commonly used" blood activating and stasis-removing" Chinese herbal drugs on adhesive function of bone marrow stroma cells in acute radiation injured mice. Methods: after irradiation by 8 Gy 60Co γ-ray, each mouse was intraperitoneally injected immediately with 0.2 ml 100% composite blood-activating decoction (CBAD) twice a day for 6 days. On the 7th day, the femura were taken and the bone karyocyte (BMC) suspension was made. According to long term bone marrow culture procedure,on the 12th day, the fibroblastic colony forming units were counted, and on the 28th day,the adhesion of culture stroma cells to normal murine BMC was observed. Results: The adhesion of culture stroma cells to normal murine BMC was 67. 8±17. 2% in normal group,while in CBAD group, it was 55. 8±10.5%. The difference was insignificant (P>0. 05).In the control group, the value was 47. 7±13. 6%, which was significantly lower than that in normal group (P<0.02). Conclusion: The commonly used" blood-activating andstasisremoving" Chinese herbal drugs can enhance the adhesive function of bone marrow stroma cells in acute radiation injured mice. |
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