| 归绥琪,俞瑾,魏美娟,杨淑萍,施大文.补肾中药对雄激素致不孕大鼠垂体、卵巢及肾上腺作用的实验研究[J].,1997,(12):735-738 |
| 补肾中药对雄激素致不孕大鼠垂体、卵巢及肾上腺作用的实验研究 |
| Experimental Study of Effect on Tonifying kidney Herbs in Pituitary Ovary Adrenal Gland or Androgen Sterilized Rats |
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| DOI： |
| 中文关键词: 补肾药 雄激素致不孕大鼠 形态学 内分泌 性腺轴 肾上腺 |
| 英文关键词:tonifying kidney herbs androgen sterilized rat morphology endocrine sex gland axis adrenal |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 目的：探讨雄激素致不孕大鼠（ＡＳＲ）发病机理和中药治疗作用。方法：采用光镜、电镜、细胞培养、免疫组化、放免测定等方法，对补肾药治疗前后的ＡＳＲ的垂体、卵巢、肾上腺的形态学及内分泌功能进行同步观察。结果：ＡＳＲ的腺垂体细胞浆内有“脂滴”、“粒溶”、“空泡”、“自嗜”、卵巢呈无排卵；肾上腺皮质网状带增生，同时促卵泡激素（ＦＳＨ）、促黄体激素（ＬＨ）明显低下（Ｐ＜０．０５～０．００５）；睾酮（Ｔ）、脱氢表雄酮（ＤＨＡ）明显升高（Ｐ＜０．０１～０．００１）。治疗后ＡＳＲ的形态学及内分泌功能均恢复正常。结论：ＳＤ雌性幼鼠９日龄给予外源性雄激素后，影响性腺轴和肾上腺，在ＡＳＲ发病机理中起重要作用；补肾药是通过对性腺轴，肾上腺多水平，多靶器官的调节，起到降低雄激素、促排卵作用。 |
| 英文摘要: |
| Objective: To observe the effect of tonifying Kidney herbs (TKH)in pituitary, ovary, adrenal gland of androgen steriled rats(ASR). Methods :ASR model was established by injecting testosterone propionate subcutaneously to SD female rats of 9 days age. Morphological and hormonal change of pituitary, ovary and adrenal gland in rats of 100 ~ 107 days old before and after feeding TKH extract were observed by light and electron microscope, cell culture immunohistochemical studies and radioimmuno-assay. Results: In the ASR groups, there were intracytopiasmic lipid drops, autophagy, vacuole, granulolysis of pituitary gland. There were anovulation apparently and incresased the amount of lipid drops in cytoplasm of interstitial glandular cell of ovary. The fatty drops of the reticular zone of adrenal gland decreased, the numbers of AGNOR (P < 0. 01 ) and PCNA(P < 0. 01 ) increased significantly. The levels of FSH, LH (P< 0. 05 ~ 0. 005)lowered, but the levels of DHA, T(P < 0. 01, 0. 05, 0. 001 ) were raised significantly. Morphological and hormonal change of all RSA returned to normal range after the treatment. Conclusions: The hyperandrogenemia were disturbed by the function of sex gland axis and adrenal in female rats of 9 days old and played an important role in pathogenesis of ASR, the TKH reduced the levels of androgen and induced ovulation through sex gland axis and adrenal level and multi-organ regulation. |
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