| 陈星,汪洛,丰美福,朱国英.雷公藤红素对大鼠血管平滑肌细胞c-myc和血小板源性生长因子的mRNA的影响[J].,1998,(3):156-158 |
| 雷公藤红素对大鼠血管平滑肌细胞c-myc和血小板源性生长因子的mRNA的影响 |
| Effect of Tripterine on mRNA Expression of c myc and Platelet Derived Growth Factor of Vascular Smooth Muscle Cell in Rats |
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| DOI： |
| 中文关键词: 雷公藤红素 血管平滑肌细胞 原癌基因 血小板源性生长因子 |
| 英文关键词:Tripterine vascular smooth muscle cell proto oncogene platelet derived growth factor |
| 基金项目:卫生部科研基金资助课题!(No.94- 1- 2 48) |
| Author Name | Affiliation | | 陈星 | Chen Xing, Wang Luo, Feng Meifu, et al Department of Cardiology, The First Affiliated Hospital of Beijing Medical University, Beijing(100034 | | 汪洛 | Chen Xing, Wang Luo, Feng Meifu, et al Department of Cardiology, The First Affiliated Hospital of Beijing Medical University, Beijing(100034 | | 丰美福 | Chen Xing, Wang Luo, Feng Meifu, et al Department of Cardiology, The First Affiliated Hospital of Beijing Medical University, Beijing(100034 | | 朱国英 | Chen Xing, Wang Luo, Feng Meifu, et al Department of Cardiology, The First Affiliated Hospital of Beijing Medical University, Beijing(100034 |
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| 中文摘要: |
| 目的 :观察雷公藤红素对大鼠血管平滑肌细胞 (VSMC)中原癌基因 (c myc)和血小板源性生长因子 (PDGF)的mRNA表达水平的影响。方法 :分别将所培养的第 5～ 1 0代VSMC同步化培养 2 4h后 ,加入 2 0 %胎牛血清 (FCS)和不同剂量的雷公藤红素 (0 1、0 2和0 3mg/L) ,共同孵育 6h和 1 2h后 ,分别提取VSMC胞浆总RNA ,通过地高辛标记探针 ,采用斑点杂交方法检测c myc和PDGF的mRNA表达。 结果 :经雷公藤红素作用后 ,VSMC的c myc和PDGF的mRNA表达较对照组明显降低 ,c mycmRNA表达水平的降低具有一定的药物剂量依赖性关系。结论 :雷公藤红素可能是通过抑制VSMC的c myc和PDGF的mRNA的表达 ,而引起抑制VSMC的过度增殖。 |
| 英文摘要: |
| Objective: To observe the effect of Tripterine on mRNA expression of c myc and platelet derived growth factor (PDGF) in vascular smooth muscle cell (VSMC) of rats. Methods: 5～10th passage culture of VSMC was used. Tripterine and 20% fetal calf serum were added into the medium of cultured VSMC in concentration of 0 1,0 2 and 0 3 mg/L after free serum cultivation for 24 hours. The general RNA was isolated from VSMC at 6 and 12 hours after the drug addition for detection of mRNA expression of c myc and PDGF respectively by dot blot hybridization. The cDNA probes were labeled by digoxin. Results: Tripterine inhibited the PDGF mRNA expression of VSMC and decreased c myc mRNA expression in a dose dependent manner, either vs. control. Conclusion: Tripterine could inhibit mRNA expression of c myc and PDGF in VSMC, therefore, it would inhibit overproliferation of VSMC. |
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