| 陈文东,安文林,楚晋,景向红,李瑞午,王惠琴,李林.茯苓水提液对新生大鼠神经细胞内钙离子浓度的影响[J].,1998,(5):293-295 |
| 茯苓水提液对新生大鼠神经细胞内钙离子浓度的影响 |
| Effect of Water Extract of Poria on Cytosolic Free Calcium Concentration in Brain Nerve Cells of Neonatal Rats |
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| DOI： |
| 中文关键词: 茯苓水提液 胞浆内钙离子 神经细胞 谷氨酸 激光共聚焦扫描显微镜 |
| 英文关键词:Poria cocos cytosolic free calcium neuron glutamate laser confocal scanning microscope |
| 基金项目:北京市科委;北京市中医药管理局资助项目!(No JX95 15 ) |
| Author Name | Affiliation | | 陈文东 | Chen Wendong, An Wenlin, Chu Jin, et al Department of Pharmacology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing (100053 | | 安文林 | Chen Wendong, An Wenlin, Chu Jin, et al Department of Pharmacology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing (100053 | | 楚晋 | Chen Wendong, An Wenlin, Chu Jin, et al Department of Pharmacology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing (100053 | | 景向红 | Chen Wendong, An Wenlin, Chu Jin, et al Department of Pharmacology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing (100053 | | 李瑞午 | Chen Wendong, An Wenlin, Chu Jin, et al Department of Pharmacology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing (100053 | | 王惠琴 | Chen Wendong, An Wenlin, Chu Jin, et al Department of Pharmacology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing (100053 | | 李林 | Chen Wendong, An Wenlin, Chu Jin, et al Department of Pharmacology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing (100053 |
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| 中文摘要: |
| 目的 :探讨茯苓水提液对神经细胞内钙稳态的影响。方法 :应用双波长荧光分光光度计和激光共聚焦扫描显微镜测定新生大鼠大脑皮层神经细胞悬液和海马神经元原代培养在加入茯苓水提液后细胞内钙离子浓度的变化。结果 :茯苓水提液在 3 1～ 2 5 0mg/L时 ,可诱导细胞内钙离子浓度升高 9 9%～ 3 3 7% ,并且这种作用随给药浓度的增大而增强 ;当浓度大于或等于 5 0 0mg/L时 ,无明显升高胞浆内钙离子浓度的作用。 3 1～ 2 0 0 0mg/L茯苓水提液均有不同程度抑制 5 0 0 μmol/L谷氨酸诱导的升钙作用。 结论 :茯苓水提液对神经细胞内钙离子浓度具有双向调节作用。 |
| 英文摘要: |
| Objective: To investigate the effect of Poria cocos, a Chinese medicinal herb, on cytosolic free calcium concentration (〔Ca 2+ 〕i) in brain nerve cells of neonatal rats. Methods: Double wave length fluorospectrophotometer and laser confocal scanning microscope were used to measure the 〔Ca 2+ 〕i in neurons. Results: The water extract of poria (31～250mg/L) induced a dose dependent increase in 〔Ca 2+ 〕i, but poria at the range of 500mg/L did not cause 〔Ca 2+ 〕i to increase. It was also shown that the water extract of poria significantly inhibited the increment of 〔Ca 2+ 〕i induced by 500μmol/L glutamate. The similar results were observed from the experiments in primarily cultured hippcampal neurons of neonatial rats by using laser confocal scanning microscope. Conclusions: The water extract of Poria cocos has the effect of bidirectional regulation on cytosolic free calcium in brain nerve cells. |
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