| 雷燕,郝钰,邱全瑛,黄启福,王永炎.复圣散对血管内皮细胞活性及其与中性粒细胞粘附率的影响[J].,1998,(10):612-615 |
| 复圣散对血管内皮细胞活性及其与中性粒细胞粘附率的影响 |
| Effect of Fusheng Powder on Activity of Vascular Endothelial Cells and Its Adhesion to Polymorphonuclear Neutrophils |
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| DOI： |
| 中文关键词: 中性粒细胞 血管内皮细胞 细胞粘附 复圣散 |
| 英文关键词:Fusheng powder polymorphonuclear neutrophil vascular endothelial cell cell adhesion |
| 基金项目:中国博士后基金会资助项目!(No.0 5 432 ) |
| Author Name | Affiliation | | 雷燕 | Lei Yan ,Hao Yu, Qiu Quanying, et al Department of Pathophysiology, Faculty of Basic Medicine, Beijing University of Traditional Chinese Medicine and Pharmacology, Beijing (100029 | | 郝钰 | Lei Yan ,Hao Yu, Qiu Quanying, et al Department of Pathophysiology, Faculty of Basic Medicine, Beijing University of Traditional Chinese Medicine and Pharmacology, Beijing (100029 | | 邱全瑛 | Lei Yan ,Hao Yu, Qiu Quanying, et al Department of Pathophysiology, Faculty of Basic Medicine, Beijing University of Traditional Chinese Medicine and Pharmacology, Beijing (100029 | | 黄启福 | Lei Yan ,Hao Yu, Qiu Quanying, et al Department of Pathophysiology, Faculty of Basic Medicine, Beijing University of Traditional Chinese Medicine and Pharmacology, Beijing (100029 | | 王永炎 | Lei Yan ,Hao Yu, Qiu Quanying, et al Department of Pathophysiology, Faculty of Basic Medicine, Beijing University of Traditional Chinese Medicine and Pharmacology, Beijing (100029 |
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| 中文摘要: |
| 目的 :探讨中性粒细胞 (PMN)与血管内皮细胞 (VEC)的粘附特性和影响因素 ,以及中药复圣散的干预作用。方法 :以培养人脐静脉内皮细胞作为靶细胞 ,观察在高脂、高糖、肿瘤坏死因子 (TNF -α)及缺氧再氧化条件下 ,对培养的VEC活性以及PMN与VEC相互粘附的影响。结果 :高脂、高糖、TNF -α和缺氧再氧化等均可抑制VEC活性 ,并显著增强PMN与VEC的相互粘附作用 ,其粘附率正常对照组为 1 0 0 % ,高脂组则为 1 67 7% ,缺氧再氧化组为1 5 1 4% ,TNF -α组为 1 3 6 7% ,高糖组为 1 2 9 6% (均P <0 0 1 )。如事先在试验体系中加入不同浓度的中药血清 ,则粘附率明显降低 ,大、小剂量均呈现有抑制粘附效应 ,并有统计学差异。结论 :中药复圣散有良好的保护VEC和抗粘附作用 ,这可能是该方防治缺血性心脑血管疾病的机制之一。 |
| 英文摘要: |
| Objective: To explore the effect of Fusheng powder on the adhesion of polymorphonuclear neutrophils (PMN) in human peripheral blood to vascular endothelial cells (VEC). Methods: Using cultured human umbilical vein cells as target cells, while being incubated with high glucose (HG, 30 mmol/L), high lipid (HL, 20%), tumor necrosis factor α (TNF α, 10%) and hypoxia (95% N 2, 5% CO 2, 37℃, 30 min, and then in the air 30 min) for 24h, respectively, the activity of VEC and the adhesive effect of PMN to VEC were surveyed. Results: It revealed that HG, HL, TNF α and hypoxia could enhance obviously the adhesion of PMN to VEC, the cell attachment rate in normal control, HG group, TNF α, hypoxia and HL after 30 minutes contact were 100%, 129.6%, 136.7%, 151.4% and 167.7% (all P<0 01, compared with control group) respectively, but high and low dosage of the Fusheng powder could significantly inhibit the adhesion of PMN to VEC.Conclusions: HG, HL, TNF α and hypoxia could all activate EC respectively and enhance the adhesion of PMN to VEC, but the Fusheng powder could protect VEC and partly block the effect of this adhesion. However, the effects of Fusheng powder on anti adhesion are of great importance in clinical cardiovascular and cerebral vascular diseases. |
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