| 周亚伟,陈可冀.藿酮胶囊诱导人体血管平滑肌细胞凋亡的实验研究[J].,1998,(11):680-683 |
| 藿酮胶囊诱导人体血管平滑肌细胞凋亡的实验研究 |
| Experimental Study of Vascular Smooth Muscle Cell Apoptosis Induced by Huotong Capsule |
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| DOI： |
| 中文关键词: 藿酮胶囊 血管平滑肌细胞 细胞凋亡 |
| 英文关键词:Huotong Capsule vascular smooth muscle cell apoptosis |
| 基金项目:国家科委生命中心博士基金资助!(No .5 96 0 6 0 13) |
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| 中文摘要: |
| 目的 :从细胞凋亡水平研究藿酮胶囊抗动脉粥样硬化的作用机理。方法 :实验培养人胚胎胸主动脉平滑肌细胞 ,用白细胞介素 1 (IL 1 )刺激平滑肌细胞建立血管平滑肌细胞(vascularsmoothmusclecell,VSMC)增生模型 ,从细胞形态学、DNA凝胶电泳、流式细胞术 3个方面观察用藿酮胶囊诱导增生的VSMC凋亡现象。结果 :透射电镜观察发现 :藿酮胶囊处理的VSMC出现细胞皱缩、染色质凝集后细胞核消失、细胞膜隆起包被细胞器形成凋亡小体(apoptoticbody)的典型凋亡形态学改变。DNA凝胶电泳提示 :经藿酮胶囊处理的VSMCDNA显示“梯子”状带纹 ,流式细胞仪可见DNA降解的亚G1 峰 (AP峰 ) ;随着藿酮胶囊浓度的增加 ,AP峰增宽、增高。空白对照组和IL 1刺激VSMC增生模型组均不见上述变化。结论 :藿酮胶囊能够诱导人体增生的VSMC凋亡。 |
| 英文摘要: |
| Objective: To explore the anti atherogenic mechanism of Huotong Capsule (HTC) on vascular smooth muscle cell (VSMC) apoptosis. Methods: VSMC proliferation model of cultured human embryonal thoracic aorta was established by stimulating VSMC with interleukin 1 (IL 1) and HTC was applied to observe the apoptosis inducing effect by transmission electron microscopy, DNA gel electrophoresis and flow cytometry. Results: Typical apoptotic cellular changes were found after HTC treatment, the VSMC shrinked, the nuclei disappeared after chromatin agglutination, cellular membrane projected and wrapped the organelle to form apoptotic body. DNA gel electrophoresis showed “ladder” strand of DNA, a special phenomenon of cellular apoptosis. And an Ap peak of degraded DNA could be seen by flow cytometry, which widened and heightened when the concentration of HTC increased. The above mentioned changes were not found in the blank control and untreated model group. Conclusion: HTC could induce apoptosis of human proliferated VSMC. |
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