| 葛璐璐,张薇,戴云,臧燕,黄纯洁.大蒜精油抑制白细胞介素-1α诱导的单核细胞-内皮细胞粘附及机理探讨[J].,1999,(3):152-154 |
| 大蒜精油抑制白细胞介素-1α诱导的单核细胞-内皮细胞粘附及机理探讨 |
| Study on Effect and Mechanism of Essential Garlic Oil in Inhibiting Monocyte Vascular Endothelial Cell Adhesion Induced by Interleukin 1α |
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| DOI： |
| 中文关键词: 大蒜精油 内皮细胞 血管细胞粘附分子－1 白细胞介素－1α 单核细胞 粘附 |
| 英文关键词:essential garlic oil endothelial cell vascular cell adhesion molecule 1 interleukin 1α monocyte adhesion |
| 基金项目: |
| Author Name | Affiliation | | 葛璐璐 | Ge Lulu, Zhang Wei, Dai Yun, et al The First Military Medical University, Guangzhou (510515 | | 张薇 | Ge Lulu, Zhang Wei, Dai Yun, et al The First Military Medical University, Guangzhou (510515 | | 戴云 | Ge Lulu, Zhang Wei, Dai Yun, et al The First Military Medical University, Guangzhou (510515 | | 臧燕 | Ge Lulu, Zhang Wei, Dai Yun, et al The First Military Medical University, Guangzhou (510515 | | 黄纯洁 | Ge Lulu, Zhang Wei, Dai Yun, et al The First Military Medical University, Guangzhou (510515 |
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| 中文摘要: |
| 目的：观察大蒜精油（ＥＧＯ）对白细胞介素－１α（ＩＬ－１α）诱导的人脐静脉内皮细胞（ＨＵＶＥＣ）上血管细胞粘附分子－１（ＶＣＡＭ－１）表达及单核细胞粘附率的影响。方法：胰酶消化法分离ＨＵＶＥＣ，用Ｕ９３７单核细胞与之联合培养，分别加入ＩＬ－１α和ＥＧＯ加ＩＬ－１α孵育，计算单核细胞－内皮细胞粘附率，ＶＣＡＭ－１免疫荧光染色，粘附细胞仪（ＡＣＡＳ５７０）测定平均荧光值。结果：单独培养及联合培养的情况下，ＥＧＯ均对ＩＬ－１α诱导的ＨＵＶＥＣ上ＶＣＡＭ－１表达具有明显的抑制作用，并能显著下调ＩＬ－１α作用下单核细胞 内皮细胞的粘附。结论：ＥＧＯ具有抗单核细胞与血管内皮细胞粘附的作用，其机理可能是通过抑制内皮细胞表面粘附分子的表达。 |
| 英文摘要: |
| Objective: To observe the effects of essential garlic oil (EGO) on vascular cell adhesive molecule 1 (VCAM 1) expression of endothelial cell and monocyte adhesion induced by interleukin-1α (IL-1α). Methods: Human umbilical vein endothelial cells (HUVEC) were isolated by pancreatin digestion method and co cultured with U937 monocyte and IL-1α or EGO+IL-1α respectively. The adhesion rate of monocyte to endothelial cell was measured and mean fluorescent intensity of immuno stained of VCAM 1 was determined by ACAS 570. Results: EGO showed significant inhibition on VCAM 1 expression induced by IL-1α either in simple culture with HUVEC or in co culture with HUVEC+U937, and could obviously down regulate the monocyte endothelial cell adhesion under effect of IL-1α. Conclusion: EGO has the effect of antagonizing against adhesion of monocyte and vascular endothelial cell, it may be due to the inhibition of EGO on adhesive molecular expression on endothelial cell. |
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