孙斐,俞瑾,张利能,查锡良.中药天癸方对雄激素致不孕大鼠下丘脑Leptin受体及神经肽Y mRNA的影响[J].,2000,(5):362-364 |
中药天癸方对雄激素致不孕大鼠下丘脑Leptin受体及神经肽Y mRNA的影响 |
Effect of Tiangui Recipe on Expressions of Hypothalamic Leptin Receptor and Messenger Ribonucleic Acid of Neuropeptide Y in Androgen-Sterilized Rats |
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DOI: |
中文关键词: 天癸方 雄激素致不孕大鼠 神经肽Y leptin受体 信使核糖核酸 原位杂交 |
英文关键词:Tiangui Recipe androgen-sterilized rat neuropeptide Y leptin receptor messenger ribonucleic acid in situs hybridization |
基金项目:上海市医学领先专业重点学科中西医结合妇科资助!96001 |
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中文摘要: |
目的:从基因转录水平探讨中药天癸方对雄激素致不孕大鼠(ASR)肥胖及无排卵的影响。方法:采用α-32P标记瘦素(leptin)受体(OB-R)及神经肽Y(NPY)寡核苷酸探针原位杂交、放射自显影技术结合图像分析,观察中药治疗前后下丘脑弓状核(AR)两种物质mRNA的表达变化。结果:ASR中ARC的NPY基因表达水平明显增加(P<0.01),OB-R基因表达水平明显降低(P<0.01),ASR呈肥胖、无排卵状态。用中药天癸方治疗后,NPY基因表达下降,OB-R基因表达上升至正常大鼠水平。结论:ASR肥胖及无排卵可能与NPYmRNA过度表达、OB-RmRNA数目异常有关,中药可调节NPY和OB-RmRNA表达而发挥减肥及促排卵作用。 |
英文摘要: |
To explore the effect of Tiangui Recipe (TGR) on obesity and anovulation in androgen-sterilized rats (ASR) from the gene transcription level. Methods: In situs hybridization and autoradiography were adopted, using α-32 P-radiolabelled leptin receptor (OB-R) and neuropeptide Y (NPY) oligonucleotides probes and integrating with image pattern analysis, to observe the effect of TGR on changes of OB-R, NPY mRNA expression in the hypothalamic arcuate nucleus (ARC). Results: Compared to the normal rats, the levels of hypothalamic OB-R mRNA expression decreased, NPY mRNA expression elevated significantly in ASR (P < 0.01 ).ASR characterized by obesity and anovulation. There was no significant difference in hypothalamic OB-R, NPY mRNA expressions between normal rats and ASR with TGR administration. Conclusion: The increased hypothalamic NPY and decreased OB-R mRNA expression may be important contributing factors to the development of obesity and anovulation in ASR. TGR may play a role in reducing weight and inducing ovulation by regulating NPY/OB-R mRNA expression. |
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