| 王莉,俞瑾.中药天癸方对雄激素致不孕大鼠下丘脑前阿黑皮原mRNA的影响[J].,2001,(7):519-521 |
| 中药天癸方对雄激素致不孕大鼠下丘脑前阿黑皮原mRNA的影响 |
| Effect of Tiangui Recipe on Hypothalamic Proopiomelanocortin mRNA Levels in Androgen Sterilized Rats |
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| DOI： |
| 中文关键词: 天癸方 雄激素致不孕大鼠 雄激素受体 前阿黑皮原mRNA |
| 英文关键词:Tiangui recipe androgen sterilized rats androgen receptor proopiomelanocortin mRNA |
| 基金项目:上海市医学领先专业重点学科中西医结合妇科资助项目 (No .960 0 1 ) |
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| 中文摘要: |
| 目的 :探讨中药天癸方对雄激素致不孕大鼠 (ASR)的作用机理。方法 :取新生SD雌性大鼠随机分为 3组 ,于 9日龄时注射丙酸睾丸酮 ,制成ASR模型 ,用中药天癸方治疗 14天 ,于 10 6日龄左右 (动情前期 )处死 ,并取血清 ,用放免法测定总睾酮 (TT)、游离睾酮 (FT) ,下丘脑前阿黑皮原mRNA(POMCmR NA)。结果 :模型组大鼠均无排卵 ,对照组 (未造模 )大鼠均排卵 ,中药组大鼠排卵率为 58%。TT和FT模型组均明显高于对照组和中药组 (P <0 0 1)。 3组大鼠在下丘脑弓状核神经元雄激素受体 (AR)和 β 内啡肽( β EP)共存。下丘脑POMCmRNA水平模型组高于对照组 (P <0 0 1) ,中药组降至基本正常。 结论 :ASR模型的血清雄激素水平升高 ,有可能通过下丘脑β EP神经元上的AR引起POMCmRNA表达增加 ,中药天癸方可使血清雄激素水平和下丘脑POMCmRNA下降 |
| 英文摘要: |
| To explore the effect of Tiangui recipe (TGR) in androgen sterilized rats (ASR). Methods: New born female SD rats were randomly divided into 3 groups. ASR model was established by means of testosterone injection subcutaneously at the age of 9 days, ASR were administered with TGR for 14 days. Around the age of 106 days (proestrus day), all rats were sacrificed, serum total testosterone (TT) and free testosterone (FT) were measured by radioimmunoassay (RIA), and levels of hypothalamic proopiomelanocortin (POMC) mRNA expression in hypothalamus were determined. Results: None of the ASR ovulated and all rats in control group ovulated. The rate of ovulation in TGR group was 58%, the mean serum concentration of TT and FT in ASR were significantly higher than those in the control group and TGR group (P<0 01). The hypothalamic POMC mRNA level in ASR model group was significantly higher than that in the control group (P<0 01), and became normal after TGR treatment. Conclusion: The elevated serum androgen levels in ASR could challenge hypothalamic POMC mRNA overexpression via androgen receptor (AR). TGR might reduce serum androgen and lower the expression of POMC mRNA. |
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