| 董莉,李斌,章云,韩志芬,王振宜.生肌化瘀方及其拆方对大鼠创面成纤维细胞Ⅰ、Ⅲ型胶原合成的影响[J].,2002,(3):200-202 |
| 生肌化瘀方及其拆方对大鼠创面成纤维细胞Ⅰ、Ⅲ型胶原合成的影响 |
| Effect of Shengji Huayu Recipe and Its Disassembled Formulae on TypeⅠandⅢCollagen Synthesis in Wound Healing Fibroblasts |
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| DOI： |
| 中文关键词: 生肌化瘀方 拆方 药物血清 成纤维细胞Ⅰ、Ⅲ型胶原 |
| 英文关键词:Shengji Huayu Recipe disassembled formulae drug containing serum fibroblast collagen type Ⅰ and Ⅲ |
| 基金项目:国家自然科学基金资助项目 (No .3990 0 1 95);上海市高等学校青年科学基金课题 (No.98QN71 ) |
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| 中文摘要: |
| 目的 :探讨生肌化瘀方及其拆方促进创面修复 (呈皮肤修复 )的作用机理。方法 :采用体外培养创面肉芽组织成纤维细胞 ,以乳鼠皮肤成纤维细胞作对照 ,分别加入生肌方、化瘀方及生肌化瘀方大、小剂量药物血清 ,运用细胞化学ABC法检测成纤维细胞Ⅰ、Ⅲ型胶原的含量。结果 :生肌方能够提高创面成纤维细胞Ⅰ、Ⅲ型胶原含量 ,且均高于模型组 (P <0 0 1) ;化瘀方能够降低创面成纤维细胞Ⅰ、Ⅲ型胶原含量 ,且低于模型组 (P <0 0 1) ;生肌化瘀方大、小剂量组Ⅰ、Ⅲ型胶原含量与正常组比较差异均无显著性 (P >0 0 5 )。结论 :生肌化瘀方能够促进创面修复 ,其可能的作用机理是通过调节Ⅰ、Ⅲ型胶原的比值来调控Ⅰ、Ⅲ型胶原代谢 |
| 英文摘要: |
| Objective: To explore the mechanism of Shengji Huayu Recipe (SJHYR) and its 3 disassembled formulae in accelerating wound healing toward skin restoration. Methods: Using in vitro cultured fibroblasts from granulation tissue of wound and compared with the normal skin fibroblast of suckling rat, who were treated separately by drug serum containing high and low dose SJHYR and its disassembled prescriptions (Shengji formula and Huayu formula). The type Ⅰ and Ⅲ collagen contents in the fibroblasts were determined with immunocytochemical ABC method. Results: Shengji formula could increase the levels of type Ⅰ and Ⅲ collagens in fibroblasts, which was significantly higher than those in model cells and control (P<0 01). Huayu formula lowered them to the levels below those in model cells (P<0 01), while in comparing the levels in the cells treated by high or low dose SJHYR with those in control, no significant difference was shown(P>0 05). Conclusion: SJHYR might accelerate wound healing toward skin restoration through regulating the ratio of collagen type Ⅰ and Ⅲ, to adjust their metabolism. |
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