| 尹飞,杨于嘉,虞佩兰,俞燕.黄芩甙对百日咳菌液致离体大鼠脑组织损害的保护作用及量效关系的研究[J].,2002,(4):286-288 |
| 黄芩甙对百日咳菌液致离体大鼠脑组织损害的保护作用及量效关系的研究 |
| Study of Protective Effect and Mechanism for Baicalin on Bacillus Pertussis Infected Brain Tissue and Its Dose-Effect Relationship |
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| DOI： |
| 中文关键词: 黄芩甙 脑组织切片 谷氨酸 过氧化氢 百日咳菌 |
| 英文关键词:baicalin brain tissue slice glutamic acid hydrogen peroxide Bacillus pertussis |
| 基金项目:国家自然科学基金资助 (No .39870 2 51 ) |
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| 中文摘要: |
| 目的 :了解黄芩甙对百日咳菌液致离体大鼠脑组织损害的保护作用、量效关系及可能的机制。方法 :大鼠脑组织切片分为 7组 :(1)正常脑组织切片培养组 (正常组 ) ;(2 ) 10 %百日咳菌液脑组织切片培养组 (模型组 ) ;(3)黄芩甙预处理后 10 %百日咳菌液脑组织切片培养组 (黄芩甙组 ) ,根据预处理黄芩甙的不同剂量又将本组分为 5个亚组 ;(4)谷氨酸脑组织切片培养组 (谷氨酸组 ) ;(5 )黄芩甙预处理后谷氨酸脑组织切片培养组 (黄芩甙加谷氨酸组 ) ;(6 )过氧化氢 (H2 O2 )脑组织切片培养组 (过氧化氢组 ) ;(7)黄芩甙预处理后过氧化氢脑组织切片培养组 (黄芩甙加过氧化氢组 )。收集各组脑组织切片培养上清液测定乳酸脱氢酶(LDH)含量。脑切片组织作蛋白质 (pro)定量检测。结果 :模型组、谷氨酸组和过氧化氢组的LDH释放量明显升高 ,分别依次为 (15 10± 4 89)、(15 4 9± 5 6 6 )、(16 5 4± 5 4 7)u/g .pro ,与正常组 (6 10± 2 87)u/g .pro比较差异有显著性 (P <0 0 1) ;0 2 5mmol/L黄芩甙预处理后 ,LDH释放量〔(8 6 5± 2 4 3)u/g .pro〕明显降低 ,与模型组比较差异有显著性 (P <0 0 1)。同时黄芩甙加谷氨酸组〔(9 93± 2 89)u/g .pro〕和黄芩甙加过氧化氢组〔(9 5 4± 2 82 )u/g .pro〕培养液中的L |
| 英文摘要: |
| Objective: To study the possible mechanism of protective effect for Baicalin on Bacillus pertussis (BP) infected brain tissue and the dose effect relationship. Methods: Brain tissues slices were divided into 7 groups: (1) the normal group; (2) the model group: infected by 10% BP; (3) the baicalin group, which was pretreated with baicalin, infected by BP and subdivided into 5 sub groups according to different doses of baicalin used; (4) the glutamic acid group: cultured with glutamic acid; (5) the baicalin plus glutamic acid group; (6) the peroxide group: cultured with hydrogen peroxide; and (7) the baicalin plus peroxide group. The lactate dehydrogenase (LDH) content in the supernatant of culture was determined and quantitative protein determination was conducted. Results: The LDH releasing was higher in the model group, glutamic acid group and peroxide group as compared with that in the normal group, 15.10±4 89 u/g. protein (the same unit below), 15.49±5.66 and 16.54±5.47 vs 6.10±2.87 respectively (P<0 01). After being pretreated with 0.25 mmol/L baicalin, LDH level decreased significantly to 8.65±2.43, which was significantly different from that in the model group (P<0 01), LDH was also decreased in the baicalin plus glutamic acid group (9.93±2.89) and baicalin plus peroxide group (9.54±2.82), which was significantly lower than that in the glutamic acid group and the peroxide group respectively (P<0 01). Conclusion: Pretreatment of baicalin has protective effect on BP caused nerve cell injury in rat brain slices, the protection is possibly related with the reduction of glutamic acid and hydrogen peroxide induced damage on nerve cells in vitro. |
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