| 裘莹,李永渝,厉曙光,宋伯根,赵桂芬.清胰汤对急性胰腺炎大鼠胰腺及肝细胞钙-镁ATP酶的影响[J].,2003,(2):112-115 |
| 清胰汤对急性胰腺炎大鼠胰腺及肝细胞钙-镁ATP酶的影响 |
| Effect of Qingyi Decoction on Ca 2+ -Mg2+ ATPase in Pancreatic and Liver Cells of Rats with Acute Pancreatitis |
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| DOI： |
| 中文关键词: 急性胰腺炎 清胰汤 钙-镁ATP酶 酶组织化学 逆转录聚合酶链反应 |
| 英文关键词:acute pancreatitis Qingyi Decoction Ca 2+ -Mg 2+ ATPase enzyme histochemistry RT PCR |
| 基金项目:国家自然科学基金资助项目 (No .30 0 60 0 31 ) |
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| 中文摘要: |
| 目的 :研究急性胰腺炎 (AP)大鼠胰腺及肝细胞钙 -镁ATP酶的变化及清胰汤等药物对其影响 ,进一步探讨这些药物治疗AP的作用机制。方法 :将 81只SD大鼠分为正常对照组、AP组、AP后清胰汤(AP +清胰汤 )治疗组及粉防己碱 (Tet ,AP +Tet)治疗组共 4组 ,在制备AP动物模型后 1h、5h、10h活体取胰、肝组织 ,通过钙 -镁ATP酶的酶组织化学染色检测其活性 ;同时通过逆转录聚合酶链反应 (RT PCR) ,测定其组织钙 -镁ATP酶的表达。结果 :钙 -镁ATP酶酶组织化学染色阳性率AP组低于正常对照组(P <0 0 5 ) ,且AP组 5h的阳性率低于 1h ;清胰汤、AP +Tet组的高于AP组 (P <0 0 5 ) ,且 5h的阳性率高于 1h。RT PCR结果显示 :胰腺组织钙 -镁ATP酶的表达AP组最低 ,且AP组 5h的表达低于 1h ;AP +清胰汤、AP +Tet组的表达高于AP组 ,且 5h的表达高于 1h ;肝组织钙 -镁ATP酶的表达各组均为阳性 ,各组间差异无显著性。结论 :AP时大鼠胰腺及细胞钙 -镁ATP酶活性降低 ,清胰汤、Tet可提高细胞钙 -镁ATP酶活性 ,减轻细胞钙超载。 |
| 英文摘要: |
| Objective: To study the variation of intracellular Ca 2+ -Mg 2+ ATPase activity in acute pancreatitis (AP) rats and the effect of Qingyi Decoction (QYD) on it, so as to explore the mechanism of QYD. Methods: Eighty one SD rats were divided into normal group, AP group, QYD group and Tet group (treated with tetrandrine). Pancreas and liver tissues were taken out 1hr, 5 hrs and 10 hrs after modelling to determine the Ca 2+ Mg 2+ ATPase activity by enzyme histochemical staining and the Ca 2+ -Mg 2+ ATPase expression by RT PCR. Results: The Ca 2+ -Mg 2+ ATPase positive rate in the AP group was lower than that in the normal group (P<0 05), and that in AP group 5 hrs after modelling was lower than that 1 hr after modelling. While in the two treated groups, Ca 2+ -Mg 2+ ATPase positive rate was higher than that in the AP group, with higher level at 5 hrs than at 1 hr after modelling. Ca 2+ -Mg 2+ ATPase expression was found positive in all the groups with insignificant difference. Conclusion: Ca 2+ -Mg 2+ ATPase activity lowered during AP, which may take part in the pathogenesis and development of cell calcium overload in AP and lead to the destruction of normal cellular structure and functions, so as to exacerbate the pathologic changes. QYD and Tet could raise the activity of Ca 2+ -Mg 2+ ATPase and reduce the cell calcium overload in the process of AP treatment. |
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