| 田国庆,郭赛珊,卢贺起.补肾活血方对缺血再灌注脑损伤小鼠学习记忆功能及海马神经细胞凋亡的影响[J].,2003,(2):123-127 |
| 补肾活血方对缺血再灌注脑损伤小鼠学习记忆功能及海马神经细胞凋亡的影响 |
| Effect of Bushen Huoxue Recipe on Learning and Memory Dysfunction and Hippocampal Nerve Cell Apoptosis in Mice with Cerebral Ischemia/Reperfusion Injury |
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| DOI： |
| 中文关键词: 补肾活血方 学习记忆 缺血再灌注 神经细胞 凋亡 |
| 英文关键词:Bushen Huoxue Recipe learning and memory ischemia/reperfusion nerve cell apoptosis |
| 基金项目:国家中医药管理局科研基金 (No .97A2 0 1 ) |
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| 中文摘要: |
| 目的 :观察补肾活血方对缺血再灌注脑损伤小鼠学习记忆功能及海马神经细胞凋亡的影响。方法 :采用双侧颈总动脉夹闭反复缺血再灌术 ,建立脑缺血学习记忆障碍及海马神经细胞凋亡小鼠模型。以Morris水迷宫测试小鼠学习记忆功能 ;HE及尼氏染色光镜观察皮层及海马病理形态改变 ;碘化丙啶 (PI)与Hoechst 33342及流式细胞仪检测凋亡细胞 ,与阿尼西坦及灯盏花注射液对照 ,观察小鼠学习记忆功能及海马神经细胞凋亡的变化及补肾活血方对其影响。结果 :模型组小鼠记忆能力潜伏期较正常对照组及假手术组延长 (P <0 0 5 ) ;皮层及海马神经细胞出现退化、变性、坏死等病理改变 ;海马神经细胞凋亡百分率升高 ,术后 6h达到高峰 (P <0 0 1)。补肾活血方能缩短小鼠潜伏期 (P <0 0 5 ) ;降低海马神经细胞凋亡百分率 ,与模型组比较 ,差异有显著性 (P <0 0 5 )。结论 :补肾活血方具有改善缺血再灌注脑损伤小鼠学习记忆功能及减轻缺血再灌注后海马神经细胞凋亡的作用。 |
| 英文摘要: |
| Objective: To investigate the effect of Bushen Huoxue Recipe (BHR) on learning and memory dysfunction and hippocampal nerve cell apoptosis in mice with cerebral ischemia/reperfusion injury. Methods: Mice model of cerebral ischemia/reperfusion injury was established by bilateral common carotid artery blocking. The learning and memory functions were tested by Morris water maze, pathological changes of cortex and hippocampus were observed with HE staining and Nissl′s staining light microscope, morphology of apoptotic nerve cells was observed by fluorescence microscope and percentage of apoptotic cells was measured by flow cytometry. Results: The latent period of Morris water maze test in the model group was longer than that in the normal control and sham operated group (P<0 05). Pathological changes such as cortical and hippocampal nerve cell regression, degeneration, and necrosis were found in the model mice, increase of hippocampal cell apoptosis rate reached to the peak after 6 hrs of operation (P<0 01). BHR could shorten the latent period (P<0 05), lower the apoptosis rate significantly as compared with those in the model group (P<0 05). Conclusion: BHR has the effects of improving the learning and memory functions, and of reducing the hippocampal cell apoptosis rate in mice with ischemia/reperfusion injury. |
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