| 田占庄,赵宏,陈伯英.滋阴泻火中药对性早熟模型大鼠促性腺激素释放激素及其受体mRNA表达的影响[J].,2003,(9):695-698 |
| 滋阴泻火中药对性早熟模型大鼠促性腺激素释放激素及其受体mRNA表达的影响 |
| Effect of Chinese Herbal Medicine for Nourishing Yin and Purging Fire on mRNA Expressions of Gonadotropin-releasing Hormone and Its Receptor in Precocious Puberty Model Rats |
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| DOI： |
| 中文关键词: 滋阴泻火中药 促性腺激素释放激素 促性腺激素释放激素受体 性早熟 |
| 英文关键词:Chinese herbal medicine for nourishing Yin and purging Fire precocious puberty gonadotropin releasing hormone gonadotropin releasing hormone receptor |
| 基金项目:国家教委高等学校博士学科点专项科研基金资助 (No.2 0 0 1 0 2 4 60 0 8) |
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| 中文摘要: |
| 目的 :观察滋阴泻火中药对达那唑诱导的性早熟模型大鼠下丘脑促性腺激素释放激素 (GnRH)和垂体GnRH受体mRNA表达的影响 ,探讨滋阴泻火中药的作用机制。方法 :将动物分为正常组、模型组、生理盐水组和中药组。模型组、生理盐水组和中药组动物 5日龄时皮下注射达那唑 30 0 μg ;中药组在 15日龄时开始喂饲滋阴泻火中药 ,生理盐水组同时给予等量的生理盐水。免疫组织化学ABC法观察大鼠下丘脑GnRH的表达 ;逆转录聚合酶链式反应 (RT PCR)观察下丘脑GnRHmRNA和垂体GnRH受体mRNA的表达。结果 :和正常组比较 ,模型组和生理盐水组大鼠阴门开启和建立性周期的时间提前 ,下丘脑GnRHmR NA和垂体GnRH受体mRNA的表达升高 ,下丘脑GnRH免疫阳性神经元减少 ;中药组大鼠阴门开启和建立性周期的时间延缓 ,下丘脑GnRHmRNA和垂体GnRH受体mRNA的表达降低 ,下丘脑GnRH免疫阳性神经元增多。结论 :滋阴泻火中药可能通过抑制下丘脑GnRH的合成和释放以及降低垂体对GnRH的反应性 ,抑制提前启动的下丘脑 -垂体 -性腺轴功能 ,这可能是滋阴泻火中药治疗性早熟的机理。 |
| 英文摘要: |
| Objective: To probe the mechanism of Chinese herbal medicine (CHM) for nourishing Yin and purging Fire on the expressions of gonadotropin-release hormone (GnRH) and its mRNA expression in hypothalamus and GnRH receptor mRNA in pituitary in danazol induced precocious puberty model rats. Methods: Rats were divided into the normal group, the model group, the blank control group and the CHM group. Rats, except that in the normal grup, were subcutaneously administered danazol 300 μg at 5 days of age individually and CHM was fed to rats in the CHM group from 15 days of age, in the meantime, normal saline was fed to rats in the blank control group. Expression of GnRH in hypothalamus was observed by immunohistochemical method and expressions of GnRH mRNA in hypothalamus and GnRH receptor mRNA in pituitary were determined by RT-PCR. Results: Compared with rats in the normal groups, the vaginal opening and the onset of first estrus were ahead of time, the number of GnRH immunoreactive positive cells decreased and the expressions of GnRH mRNA in hypothalamus and GnRH receptor mRNA in pituitary up-regulated in the model rats and blank control rats. Compared with the model and the blank control groups, in CHM group, all the above-mentioned abnormally changed parameters improved significantly after treatment. Conclusion: CHM for nourishing Yin and purging Fire may inhibit the abnormal hyperfunction of hypothalamus-pituitary-ovary axis in precocious puberty rat induced by danazol via reducing the synthesis and release of GnRH, and lowering the responsibility of pituitary cells to GnRH. This may be the primary mechanism of CHM in effectively treating the true precocious puberty. |
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