| 陈更新,劳绍贤,黄志新,胡玲,胡斌,张诗军,黄列平,罗琦.中药对脾胃湿热证患者胃黏膜水通道蛋白3、4基因表达的影响[J].,2005,(3):199-202 |
| 中药对脾胃湿热证患者胃黏膜水通道蛋白3、4基因表达的影响 |
| Effect of Chinese Herbs on Expression of Aquaporin 3,4 Gene in Gastric Mucosa of Patients with Pi-Wei Damp-Heat Syndrome |
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| DOI： |
| 中文关键词: 清热化湿方 脾胃湿热证 慢性浅表性胃炎 水通道蛋白3、4 |
| 英文关键词:Qingre Huashi Recipe Pi-Wei Damp-Heat Syndrome chronic superficial gastritis aquaporin 3 aquaporin 4 |
| 基金项目:国家自然科学基金项目资助课题 (No .30 2 71 572 ) |
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| 中文摘要: |
| 目的探讨脾胃湿热证与胃黏膜水通道蛋白 (AQP) 3、4基因表达的关系及中药清热化湿方对其影响。方法选择慢性浅表性胃炎患者 6 8例 ,属脾胃湿热证者 5 3例 (其中湿重于热 19例 ,热重于湿 14例 ,湿热并重 2 0例 ) ,脾虚证者 15例。脾胃湿热证患者予中药清热化湿方治疗。采用荧光定量PCR方法检测胃黏膜AQP 3、4的基因表达。结果脾胃湿热证组患者AQP 3基因表达的水平高于健康人组 (10名 )和脾虚证组 ,但 3者比较 ,差异无显著性 ;AQP 4基因表达的水平则显著高于健康人组和脾虚证组 (P <0 .0 5或P <0 .0 1) ;脾虚证组与健康人组比较差异无显著性 ;脾胃湿热证各亚型之间比较呈湿重于热 >湿热并重 >热重于湿的趋势 ;治疗后脾胃湿热证患者及湿重于热亚型AQP 3、4的基因表达水平显著下降 (P <0 0 1) ,接近健康人组及脾虚证组水平。结论AQP 3、4的异常表达可能是脾胃湿热证的发生机制之一 ,中药可能通过影响AQP 3、4的表达而发挥治疗作用 |
| 英文摘要: |
| To explore the relationship between Pi-Wei Damp-Heat Syndrome (PWDHS) with expression of aquaporin (AQP) 3,4 gene in gastric mucosa and the effects of Qingre Huashi Recipe (QHR) on the expression.MethodsSixty-eight patients with chronic superficial gastritis were differentiated into Pi-Wei Damp-Heat Syndrome group (PWDHS, n=53, 19 cases with predominant Dampness, 14 cases with predominant Heat, 20 cases with Dampness equal to Heat) and Pi deficiency Syndrome group (PDS, n=15). The PWDHS were treated with QHR. The expression of AQP 3,4 gene in the two groups were determined by fluorescence quantitative polymerase chain reaction (FQ-PCR). ResultsExpression of AQP 3 gene in PWDHS was higher than that in PDS and the healthy group, but the difference showed no statistical significance. Expression of AQP 4 gene in PWDHS was obvious higher than that in PDS and the healthy group (P<0.05 or P<0.01), but the difference of AQP 4 gene expression between PDS and the healthy group was insignificant. Comparison among various sub-types of PWDHS showed that the AQP 4 gene expression in the predominant dampness > dampness (equal) to heat> predominant heat. AQP 3,4 gene expression in PWDHS was significantly decreased after QHR treatment, especially in the cases with predominant dampness syndrome (P<0.01), approaching that in the healthy group and PDS. ConclusionAbnormal expression of AQP 3,4 gene may be one of the possible mechanisms of PWDHS pathogenesis, Chinese herbs could influence AQP 3,4 gene expression to play a key role in treatment. |
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