| 殷惠军,张颖,蒋跃绒,罗兰,刘颖,史大卓,陈可冀.西洋参叶总皂苷对急性心肌梗死大鼠心肌细胞凋亡及凋亡相关基因表达的影响[J].,2005,(3):232-235 |
| 西洋参叶总皂苷对急性心肌梗死大鼠心肌细胞凋亡及凋亡相关基因表达的影响 |
| Effect of Folium Panax Quinquefolium Saponins on Apoptosis of Cardiac Muscle Cells and Apoptosis-related Gene Expression in Rats with Acute Myocardial Infarction |
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| DOI： |
| 中文关键词: 西洋参叶总皂苷 急性心肌梗死 心肌细胞凋亡 Bcl-2蛋白 Fas蛋白 |
| 英文关键词:Folium Panax quinquefolium saponins acute myocardial infarction apoptosis of cardiac muscle cells Bcl-2 protein Fas protein |
| 基金项目:首都医学发展科研基金资助 (No .0 3 Ⅲ 0 6) |
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| 中文摘要: |
| 目的观察西洋参叶总皂苷 (PQS)对急性心肌梗死大鼠心肌细胞凋亡及凋亡相关基因表达的影响。方法取Wistar大鼠 5 0只 ,随机分为 5组 ,除假手术组 10只大鼠开胸暴露左冠状动脉 ,但不作结扎外 ,其余 4 0只大鼠结扎冠状动脉左前降支造成急性心肌梗死 (AMI)模型 ,造模成功后 ,分别给PQS(大、小剂量 ,即 5 4mg/kg和 2 7mg/kg)、开搏通和生理盐水 ,连续给药 7天 ;采用原位末端标记凋亡的心肌细胞 ,免疫组织化学法测定Bcl 2和Fas的表达。结果模型组大鼠心肌细胞凋亡指数 (46 .4 8% )明显高于假手术组(1.0 3% ,P <0.0 1) ;与模型组比较 ,开搏通组和PQS大、小剂量组 3组心肌细胞凋亡显著下降 (分别为2 3.5 3%、17.5 8%、2 5. 17% ,均P <0.0 1) ;开搏通组和PQS大、小剂量组Bcl-2基因表达均上调 ,Fas蛋白表达均下调。结论PQS能明显抑制急性心肌梗死后大鼠心肌细胞凋亡 ,下调Fas蛋白表达 ,上调Bcl-2蛋白表达 ,具有抗缺血心肌损伤的作用 |
| 英文摘要: |
| observe the effect of Folium Panax quinquefolium saponins (PQS) on apoptosis of cardiac muscle cells (CMCs) and apoptosis-related gene expression in rats with acute myocardial infarction (AMI). MethodsFifty Wistar rats were randomly divided into 5 groups, 40 rats underwent coronary ligation of left anterior descending branch to establish AMI rats model, while to the other 10 rats in the sham-operation group, thoracotomy without coronary ligation was carried out. The AMI model rats in treated groups were treated with high-dosage PQS, low-dosage PQS, captopril respectively for 7 days, and those in the control group were treated with normal saline instead. Then, the apoptotic CMCs were labeled by TUNEL and the expression of Bcl-2 and Fas in cardiac muscle cells were determined by immunohistochemical methods. ResultsThe apoptotic index of CMCs in the model rats (46.48%) was significantly higher than that in the sham-operation group (1.03%, P<0.01). CMCs apoptosis rate in the low-dosage PQS group, the large-dosage group and the captopril group was 23.53%, 17.58% and 25.17%, respectively, all were significantly lower than that in the sham-operation group (P<0.01). The expression of Bcl-2 gene was up-regulated and expression of Fas protein was down-regulated in the three treated groups. ConclusionPQS can inhibit CMCs apoptosis in AMI rats, down-regulate Fas protein expression and up-regulate Bcl-2 protein expression, and has antagonistic effect in myocardial ischemic injury. |
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