| 熊宗斌,吴萍,黄云峰,张力,黄艳君,袁萍,叶笃筠.丹参改善小鼠慢性酒精性肝损伤机制的研究[J].,2005,(5):425-428 |
| 丹参改善小鼠慢性酒精性肝损伤机制的研究 |
| Protective Mechanisms of Radix Salviae Miltiorrhizae against Chronic Alcoholic Liver Injury in Mice |
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| DOI： |
| 中文关键词: 丹参注射液 酒精性肝损伤 toll样受体-4 血红素氧合酶-1 |
| 英文关键词:Radix Salviae miltiorrhizae alcoholic liver injury toll-like receptor 4 hemeoxygenase-1 |
| 基金项目:国家自然科学基金资助课题(No.30100226;30200373) |
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| 中文摘要: |
| 目的探讨丹参改善小鼠慢性酒精性肝损伤的机制。方法建立小鼠慢性酒精性肝损伤模型,采用苏木素伊红(Hematoxylin eosin ,HE)染色,观察丹参干预后肝组织形态学改变,逆转录多聚酶链式反应(reversetranscriptionpolymerasechainreaction ,RT -PCR)检测肝组织toll样受体4(tolllikereceptor 4,TLR4)mRNA及血红素氧合酶1(hemeoxygenase -1,HO -1)mRNA的水平,以免疫组织化学方法检测TLR4蛋白表达水平。结果丹参能减轻酒精所引起的肝细胞脂肪变性、坏死,下调TLR4mRNA表达及HO 1mRNA表达,并能使TLR4阳性细胞数明显减少。结论丹参可通过对TLR4信号传导途径的影响来减少酒精所引起的肝损伤。 |
| 英文摘要: |
| ObjectiveTo investigate the protective mechanisms of Radix Salviae miltiorrhizae (RSM) on chronic alcoholic liver injury in mice. MethodsThe chronic alcoholic liver injury mouse model was established. The morphologic change of hepatic tissue was observed with hematoxylin-eosin (HE) staining; the levels of toll-like receptor-4 (TLR-4) mRNA in hepatic tissue and hemeoxygenase-1 (HO-1) mRNA were determined using reverse transcription polymerase chain reaction (RT-PCR) technique; and the expression of TLR-4 protein was determined by immunohistochemistry method. ResultsRSM could alleviate the fatty degeneration and adiponecrosis of hepatic cells induced by alcohol, down-regulate the expressions of TLR-4 mRNA and HO-1 mRNA, and significantly decrease the number of TLR-4 positive cells. ConclusionRSM could prevent liver injury from alcohol by way of influencing TLR-4 signal transcription. |
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