| 李剑,范维琥,敖红,黄爱民,罗心平,施海明,李勇.中药红景天对大鼠缺血心肌Flt-1、KDR及Tie-2表达的影响[J].,2005,(5):445-448 |
| 中药红景天对大鼠缺血心肌Flt-1、KDR及Tie-2表达的影响 |
| Effect of Rhodiola on Expressions of Flt-1, KDR and Tie-2 in Rats with Ischemic Myocardium |
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| DOI： |
| 中文关键词: 红景天 血管新生 血管内皮生长因子受体 |
| 英文关键词:rhodiola angiogenesis vascular endothelial growth factor receptors |
| 基金项目:上海市科委基础研究项目(No.03JC14018) |
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| 中文摘要: |
| 目的观察中药红景天对大鼠心肌梗死后心肌内血管内皮生长因子(VEGF)各受体表达的影响。方法在确定造模成功的基础上将大鼠随机分为4组:模型组、红景天组、阳性对照组及假手术组。饲养6周后处死动物,摘取心脏制作石蜡切片,进行标准免疫组化染色操作。染色后切片在IMS细胞图像分析系统中用免疫组化定量软件进行定量分析。计算阳性面积(area)和平均光密度值(OD)。观察缺血心肌VEGF受体:Flt- 1、KDR及血管生成素(angiopoietin)的受体Tie 2的表达情况。结果中药红景天可提高红景天组大鼠心肌Flt -1与Tie -2的表达,与阳性对照组、模型组比较差异有显著性(P <0 . 0 5) ;红景天组心肌局部KDR的表达较假手术组和未干预组增加(P <0 .0 5) ,但与阳性对照组、模型组比较,差异无显著性。结论中药红景天可能通过改变缺血心肌Flt -1及Tie- 2的表达,达到促进血管新生,改善心肌缺血的作用。 |
| 英文摘要: |
| ObjectiveTo investigate the effect of rhodiola on expression of vascular endothelial growth factors receptors (VEGFR) in myocardium of rats after myocardial infarction. MethodsOn the basis of successful establishment of myocardial infarction rat model, the experimental animals were divided into the model group, the rhodiola group, the positive control group and the sham-operated group, they were sacrificed after 6 weeks feeding. Their hearts were resected and embedded in paraffin to make sections with standard immunohistochemistry stain. Then the stained slices were analyzed in the IMS cell imagine analysis system using immunohistochemical quantitative analysis software. The field of vision of left ventricular myocardial tissue in three sites selected from the marginal area of infarction in each slice were determined, the mean value was then converted to positive area. Meanwhile, the mean optical density (OD) was calculated and the various expressions of VEGFR, i.e. Flt-1, KDR and angiopoietin receptor (Tie-2) were measured. ResultsThe expressions of Flt-1 and Tie-2 in myocardial tissue were significantly increased in the rhodiola treated group after treatment, showing significant difference as compared with those in the positive control group and the model group (P<0.05). The expression of KDR in myocardium after rhodiola intervention was higher than that in the sham-operated and non-intervened group (P<0.05), but insignificantly different to that in the positive control group and model group. ConclusionRhodiola could improve angiogenesis to ameliorate myocardial ischemia by regulating the expression of Flt-1 and Tie-2 in ischemic myocardium. |
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