| 张莹,石承先,李玉祥,黄平.丹参对重症急性胰腺炎大鼠诱导型一氧化氮合成酶mRNA的表达与器官损伤的影响[J].,2005,(11):1012-1015 |
| 丹参对重症急性胰腺炎大鼠诱导型一氧化氮合成酶mRNA的表达与器官损伤的影响 |
| Effect of Salviae Miltiorrhizae on mRNA Expression of Inducible Nitric Oxide Synthase and Organ Injury in Rats with Severe Acute Pancreatitis |
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| DOI： |
| 中文关键词: 丹参注射液 重症急性胰腺炎 一氧化氮合成酶mRNA 器官损伤 |
| 英文关键词:Salviae Miltiorrhizae severe acute pancreatitis nitric oxide synthase mRNA organ injury |
| 基金项目:贵州省优秀科技教育人才省长基金资助课题(黔科教办:2001—06) |
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| 中文摘要: |
| 目的探讨重症急性胰腺炎(SAP)大鼠诱导型一氧化氮合成酶(i NOS)mRNA的表达与器官损伤的关系和丹参对其影响。方法Wistar大鼠随机分为3组,模型组和丹参组均以5%牛磺胆酸钠经胰管内逆行注射复制SAP模型,丹参组则在制模后立即肌肉注射丹参注射液(每天2ml/kg),每6h1次,共给药4次;对照组仅行假手术,模型组和对照组同期给予等体积生理盐水。各组大鼠在术后24h测定血淀粉酶(AMY)、一氧化氮(NO)和腹水量,用原位杂交法和图像分析检测胰、肺、肾i NOS mRNA的表达强度,并观察其病理变化。结果血浆中AMY、NO和腹水量,模型组显著高于丹参组及对照组(P<0.05或P<0.01)。i NOS mRNA在胰、肺、肾中的表达模型组、丹参组均增高,模型组显著高于丹参组(P<0.01)。胰、肺、肾病理改变丹参组较模型组减轻。结论SAP时胰、肺、肾组织的i NOS mRNA高表达导致NO过度生成并参与胰腺及胰外器官损伤。丹参早期治疗能抑制胰、肺、肾组织的i NOS mRNA的过度表达,对胰腺、肺及肾起保护作用。 |
| 英文摘要: |
| ObjectiveTo investigate the relationship between mRNA expression of inducible nitric oxide synthase (iNOS) and organ injury, and the effect of Salviae Miltiorrhizae on iNOS mRNA in severe acute pancreas (SAP) rats.Methods Forty-five Wistar rats were randomly divided into 3 groups, the model group (MG), the Salviae Miltiorrhizae group (SMG),and the control group (CG), with 15 rats in each group. Rats in MG and SMG were established to SAP model by intraductal injection with 5% sodium taurocholate in a dose of 1.0 ml/kg, while rats in CG were merely performed sham-operation. Immediately after modeling, rats in SMG were injected with Salviae Miltiorrhizae injection (SMI) 2 ml/kg every 6 h for 4 times in total, but to rats in other two groups same volume of normal saline were administered. The level of serum amylase (AMY) , nitric oxide (NO) and the volume of ascites of rats were determined 24h after modeling, and intensity of iNOS mRNA expression in pancreas, lung and kidney were detected in the same time using in situ hybridization and image analysis. The pathologic change was observed as well. Results The volume of ascites and serum levels of AMY and NO in MG were significantly higher then those in SMG and CG (P<0.05 and P<0.01 ). The expression of iNOS mRNA in pancreas, lung and kidney increased in MG and SMG, it was significantly higher in MG than that in SMG. And the pathological change of pancreas, lung and kidney in SMG was milder than that in MG. Conclusions Over-expression of iNOS mRNA could cause excessive synthesis of NO, and lead to injury of pancreas, lung and kidney in SAP. Salviae Miltiorrhizae in early stage of the disease can inhibit the over-expression of iNOS mRNA to ameliorate the injury of the pancreas, lung and kidney. |
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