| 臧洪敏,陈君长,刘亦恒,刘润侠,王坤正.补肾活血方对大鼠成骨细胞增殖、分化及矿化的影响[J].,2005,(12):1112-1114 |
| 补肾活血方对大鼠成骨细胞增殖、分化及矿化的影响 |
| Effects of Bushen Huoxue Recipe on Proliferation, Differentiation and Mineralization of Osteoblasts in Rats |
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| DOI： |
| 中文关键词: 补肾活血方 细胞培养 成骨细胞 |
| 英文关键词:Bushen Huoxue Recipe cell culture osteoblasts |
| 基金项目: |
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| 中文摘要: |
| 目的观察中药补肾活血方对体外培养SD大鼠成骨细胞增殖、分化及矿化的影响。方法取新生的SD大鼠的颅骨,胶原酶法培养成骨细胞。应用MTT法,对硝基苯磷酸盐法(PNPP)及茜素红染色方法观察不同浓度补肾活血方对体外培养成骨细胞在24、48、72h不同时段的增殖、碱性磷酸酶(ALP)活性及矿化结节形成的影响。结果不同浓度的补肾活血方均可促进成骨细胞增殖率提高,以中、高浓度组的作用较为明显(P<0.01);不同浓度补肾活血方可使ALP活性增强(P<0.01);中、高浓度组补肾活血方矿化结节数量,显著多于对照组(P<0.01)。结论补肾活血方具有促进成骨细胞的增殖、分化及矿化的作用。 |
| 英文摘要: |
| Objective To observe the effects of Bushen Huoxue Recipe (BHR) on cell proliferation, differentiation, maturation and mineralization in osteoblasts of rats cultured in vitro. Methods Osteoblasts from cranium of newborn SD rats were cultured by collagenase method. MTT, PNPP and ARS were used to observe the proliferation, activity of alkaline phosphatase (ALP) and the formation of mineral node of cultured osteoblasts effected by different concentrations of BHR at different time points (24, 48, 72 hrs after medication). Results Different concentrations of BHR could enhance the cell proliferation rate and ALP activity, the effects of BHR in high and moderate concentration were more significant in enhancing the cell proliferation rate (P<0.01). And the number of mineral node of cultured osteoblasts treated by high and moderate concentration BHR was much more than that of the untreated osteoblasts (P<0.01). Conclusion BHR could promote the proliferation, differentiation, and mineralization of the osteoblasts. |
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