| 吴符火,刘雪梅,郭素华.养心草胶囊调血脂机制的实验研究[J].,2006,(2):131-134 |
| 养心草胶囊调血脂机制的实验研究 |
| Study on Mechanism of Yangxincao Capsule in Regulating Lipid Metabolism |
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| DOI： |
| 中文关键词: 养心草胶囊 大鼠模型 高脂血症 调节血脂 |
| 英文关键词:Yangxincao capsule rat model hyperlipidemia blood-lipid regulation |
| 基金项目:福建省科技三项费用项目(No.2001Z028) |
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| 中文摘要: |
| 目的观察养心草胶囊的调脂作用,初步探讨该药调脂机制。方法将60只SD雄性大鼠随机分为6组。正常组(不作任何处理)10只,其余50只给予高脂乳剂(1ml/100g)连续灌胃10天造模后分为5组,分别为模型组10只,阳性对照组10只,养心草胶囊高、中、低3个剂量组各10只。正常组和模型组给等体积的饮用水;养心草胶囊高、中、低3个剂量组分别给予养心草胶囊1.08g/kg、0.54g/kg及0.27g/kg,阳性对照组给予山楂精降脂片5.4mg/kg(为中剂量的等效量),连续灌胃10天。第11天起,除正常对照组外,上午继续给高脂乳剂,下午模型组给饮用水,其他组分别给药,连续灌胃10天。第21天大鼠禁食不禁水,16h后,眼眶取血测定血清脂类、脂蛋白、载脂蛋白和脂质代谢酶。结果养心草胶囊3个剂量组及阳性对照组血清总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDLC)降低,高密度脂蛋白胆固醇(HDLC)及其亚组分(HDL2C、HDL3C和HDLC/TC)比值升高。养心草胶囊高、中剂量组血清载脂蛋白A1(apoA1)升高,载脂蛋白B(apoB)降低;养心草胶囊3个剂量组卵磷脂胆固醇酰基转移酶(LCAT)和脂蛋白脂酶(LPL)活性升高,与模型组比较,差异均有显著性(P<0.05,P<0.001)。结论养心草胶囊能明显调节高脂血症大鼠脂质代谢紊乱,对脂蛋白有一定的双向调节作用。提示养心草胶囊可降低冠状动脉病变的危险性,其调脂机制初步认为与提高脂质代谢酶LPL、LCAT的活性和提高HDL2C清除胆固醇有关。 |
| 英文摘要: |
| Objective To investigate the effect and mechanism of Yangxincao Capsule （YXCC） in regulating lipids. Methods Sixty rats were randomly divided into 6 groups, the normal control group （A）, the hyperlipidemia model group （B）, the high, middle and low dose YXCC treated groups （C, D and E）, and the Shanzhajing （SZJ） treated group （F） for positive medicine control. Except for the rats in the normal control group, the other 50 were daily fed with fatty emulsion for 10 days to establish hyperlipidemic model.From the 11th day on, in the same time of continually feeding with fatty emulsion they were administered with water, high （1.08 g/kg）, middle （0.54 g/kg）, low dose （0.27 g/kg） of YXCC and SZJ （5.4 mg/kg） respectively for 10 days, while to rats in Group A equal volume of water was given. At the 21th day, after rats were fasted for 16 h, their blood was extracted from post-orbital vein to detect the level of serum lipids, lipoprotein, apolipoprotein （apo） and lipid metabolic enzyme. Results Compared with Group A, the levels of serum total cholesterol （TC）, triglyceride （TG） and low density lipoprotein-cholesterol （LDL-C） increased remarkably, and the level of high density lipoprotein-cholesterol （HDL-C） dropped obviously in Group B. While in the four treated groups the levels of TC, TG and LDL-C were significantly reduced, HDL-C and its sub-components 2 and 3 （HDL2-C and HDL3-C）, as well as the ratio of HDL-C/TC were raised. Besides, the content of apo-A1 was increased and apo-B was decreased significantly in Group C and D, activity of lecithin cholesterol acyltransferase （LCAT） and lipoprotein lipase （LPL） increased in the three YXCC treated groups, all showed statistical significance （P<0.05 or P<0.01） as compared with those in Group B. Conclusion YXCC could remarkably modulate the lipid metabolic disorder in hyperlipidemic rats, and has a certain bi-directional regulating function on lipoprotein, inferring that it could reduce the risk of occurring coronary artery diseases.The mechanism of regulating lipid metabolism might be related with the increasing activity of LCAT, LPL and eliminating of cholesterol by the elevated level of HDL2-C. |
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