| 刘智勤,蒋玉凤,王秋华,樊永平,岳晓莉,黄启福,胡以明.脑热清对内毒素性发热家兔血管内皮细胞分泌功能的影响[J].,2006,(2):144-146 |
| 脑热清对内毒素性发热家兔血管内皮细胞分泌功能的影响 |
| Effects of Naoreqing on Secretive Function of Vaso-endothelial Cells in Rabbits with Endotoxic Fever |
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| DOI： |
| 中文关键词: 发热 内毒素 内皮细胞 家兔 |
| 英文关键词:fever endotoxin vaso-endothelial cell rabbit |
| 基金项目:北京市科委课题(No.954024200) |
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| 中文摘要: |
| 目的从血管内皮细胞分泌功能探讨脑热清(NRQ)口服液对内毒素性发热家兔的作用。方法复制内毒素性发热家兔模型,观察NRQ对家兔体温的影响,采用放免法测定血浆血栓素B2(TXB2)、6酮前列腺素F1α(6ketoPGF1α)以及内皮素(endothelin,ET)的含量,用发色底物法测定血浆组织纤溶酶原激活物(tPA)和组织纤溶酶原激活物抑制剂(PAI)的活性。结果NRQ+内毒素组的ΔT[(0.69±0.07)℃]、TRI2(4.85±0.57)、ET[(197.96±39.11)ng/L]和TXB2[(177.35±77.30)ng/L]的含量以及PAI[(0.84±0.01)AU/ml]的活性明显低于内毒素组的ΔT[(1.31±0.13)℃]、TRI2(8.44±0.98)、ET[(250.80±40.99)ng/L]和TXB2[(279.64±83.74)ng/L]的含量以及PAI[(0.86±0.01)AU/ml]的活性,两者比较差异有显著性(P<0.05,P<0.01);而6ketoPGF1α[(986.70±327.36)ng/L]的含量和tPA[(0.25±0.02)IU/ml]的活性明显高于内毒素组6ketoPGF1α[(507.81±170.01)ng/L]的含量和tPA[(0.21±0.02)IU/ml]的活性,两者比较差异亦有显著性(P<0.01)。结论NRQ可能通过改善发热家兔血管内皮细胞的分泌功能,起到扩张血管,加快体表散热,从而对内毒素性发热家兔的解热效应起到一定的整合作用。 |
| 英文摘要: |
| Objective To observe the antifebrile effect of Naoreqing （NRQ） oral liquid on secretive function of vaso-endothelial cells in rabbits with endotoxic fever. Methods Endotoxic fever rabbit model was duplicated to observe the effects of NRQ on body temperature, blood levels of thromboxane B2 （TXB2）, 6-keto-prostaglandin F 1α （6-keto-PGF 1α） and endothelin （ET） using radioimmunoassay, as well as activity of tissue-type plasminogen activator （t-PA） and plasminogen activator inhibitor （PAI） in plasma by chromophoric substrate assay. Results Comparisons of various indexes between the two groups showed significantly difference, i.e. the maximal increment of body temperature: 0.69±0.07℃ vs 1.31±0.13℃ （the NRQ treated group vs the untreated model group, the same hereafter）; 2h thermal response index TRI2 4.85±0.57 vs 8.44±0.98; plasma ET content 197.96±39.11 ng/L vs 250.80±40.99 ng/L; TXB2 content 177.35±77.30ng/L vs 279.64±83.74 ng/L; activity of PAI 0.84±0.01AU/ml vs 0.86±0.01 AU/ml; plasma 6-keto-PGF 1α content 986.70±327.36 ng/L vs 507.81±170.01 ng/L; activity of t-PA 0.25±0.02 IU/ml vs 0.21±0.02 IU/ml （P<0.05,P<0.01）. Conclusion NRQ may improve secretive function of vaso-endothelial cells to dilate blood vessel and quicken heat dissipation through body surface, so as to play an integral antipyretic effect in rabbits with endotoxic fever. |
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