| 叶爱丽,陆付耳,徐丽君.黄连解毒汤对胰岛素抵抗大鼠脂肪组织胰岛素受体及其底物信号转导的影响[J].,2006,(10):909-912 |
| 黄连解毒汤对胰岛素抵抗大鼠脂肪组织胰岛素受体及其底物信号转导的影响 |
| Effects of Huanglian Jiedu Decoction on Signal Transduction of Insulin Receptor and Insulin Receptor Substrate in Adipose Tissue of Insulin Resistant Rats |
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| DOI： |
| 中文关键词: 黄连解毒汤 胰岛素受体底物1 胰岛素抵抗 |
| 英文关键词:Huanglian Jiedu Decoction insulin receptor substrate-1 insulin resistance |
| 基金项目:国家自然科学基金资助项目(No.30371816);教育部高等学校博士学科点专项科研基金课题(No.20030487008) |
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| 中文摘要: |
| 目的观察黄连解毒汤对胰岛素抵抗大鼠脂肪组织中胰岛素受体(InsR)酪氨酸磷酸化和胰岛素受体底物1(IRS-1)表达及其酪氨酸磷酸化水平的影响,探讨其改善胰岛素抵抗的分子机制。方法采用小剂量链脲佐菌素尾静脉注射加高糖高脂饲料喂养方法建立Wistar大鼠胰岛素抵抗模型,以黄连解毒汤干预治疗10周,检测血糖和血清胰岛素,用免疫沉淀和Western blot方法检测黄连解毒汤治疗后胰岛素抵抗大鼠附睾脂肪组织内InsR酪氨酸磷酸化和IRS-1蛋白表达水平及酪氨酸磷酸化水平。结果黄连解毒汤治疗胰岛素抵抗大鼠后,脂肪组织内IRS-1表达较模型组增加,InsR和IRS-1酪氨酸磷酸化水平较模型组显著增加。结论黄连解毒汤促进胰岛素抵抗大鼠脂肪组织InsR和IRS-1酪氨酸磷酸化水平的表达,这可能是其降血糖并改善组织胰岛素敏感性的机制之一。 |
| 英文摘要: |
| Objective To investigate the effects of Huanglian Jiedu decoction(HLJD)on protein expressions and tyrosine phosphorylation levels of insulin receptor(InsR)and insulin receptor substrate-1(IRS-1)in adipose tissue of insulin resistant(IR)rats,and explore its possible molecular mechanism in improving IR.Methods IR model was induced by intravenous injection of a small dose of streptozotocin combined with high fat and caloricity diet feeding in Wistar rats.And the model rats in the testing group were treated with HJD for 10 weeks.Fasting serum glucose and insulin were determined,and protein expressions and tyrosine phosphorylation levels of InsR and IRS-1 in adipose tissue of epididymides were detected by immunoprecipitation and Western blot.Results The protein expression of IRS-1 and the tyrosine phosphorylation levels of InsR and IRS-1 increased significantly in model rats treated with HLJD,compared with those in the untreated model rats.Conclusion HLJD could increase the tyrosine phosphorylation levels of InsR and IRS-1 in adipose tissue in IR rats,which maybe one of its mechanisms in lowering blood glucose and improving insulin sensitivity of the target tissues. |
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