| 吴以岭,魏聪,王宏涛,贾振华,高怀林,袁国强.通肾络胶囊对糖尿病肾病大鼠肾脏细胞外基质成分及代谢的影响[J].,2007,(4):326-330 |
| 通肾络胶囊对糖尿病肾病大鼠肾脏细胞外基质成分及代谢的影响 |
| Effect of Tongshenluo Capsule on the Components of Extracellular Matrix and Their Metabolism in Kidney of Rats with Diabetic Nephropathy |
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| DOI： |
| 中文关键词: 糖尿病肾病 通肾络胶囊 细胞外基质 成分 代谢 |
| 英文关键词:diabetic nephropathy Tongshenluo Capsule extracellular matrix component metabolism |
| 基金项目:国家重点基础研究发展计划(973计划)项目(No2005CB523301);河北省科学技术研究与发展计划资助项目(No06276101D) |
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| 中文摘要: |
| 目的观察中药通肾络胶囊对糖尿病肾病(DN)大鼠模型肾脏细胞外基质(ECM)成分及代谢的影响,探讨通肾络胶囊对DN大鼠肾脏保护作用机制。方法采用单侧肾脏切除并腹腔注射链脲佐菌素(STZ)的方法复制DN大鼠模型,分为假手术、模型、缬沙坦、糖适平及通肾络胶囊组,每组10只。动态检测各组大鼠空腹血糖(FBG)、24h尿微量白蛋白(24hmAlb),末次给药后测定大鼠血浆糖化血红蛋白(HbA1c)、透射电镜观察各组大鼠肾脏超微结构改变,应用免疫组化技术及图像采集分析系统测定各组大鼠肾脏组织中Ⅳ型胶原(Ⅳ-C)、纤维连接蛋白(FN)、层粘连蛋白(LN)的表达,测定影响ECM代谢的基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶抑制剂-2(TIMP-2)及转化生长因子β1(TGF-β1)的表达。结果通肾络胶囊可降低DN大鼠FBG、HbA1c、24hmAlb的水平(P<0.05或P<0.01),减轻肾小球基底膜增厚程度,减少ECM成分,降低TGF-β1、TIMP-2的表达,升高MMP-2的表达(P<0.05或P<0.01)。结论通肾络胶囊可能通过减少ECM成分表达、调节ECM代谢,从而发挥对DN大鼠肾脏的保护作用。 |
| 英文摘要: |
| Objective To investigate the effect of Tongshenluo Capsule (TSL) on the components of extracellular matrix (ECM) and their metabolism in kidney of rats with diabetic nephropathy (DN), and to explore its mechanism of kidney protecting. Methods The DN model rats established by one side nephrectomy and intraperitoneal injection of streptozotocin were randomly divided into 5 groups, the sham-operation group, the model group, the Valsartan group, the Gliquidon group, and the TSL group, 10 in each group. Levels of fasting blood glucose (FBG) and 24-h urinary micro-content of albumin (24 h mAlb) were determined dynamically; the serum glycosyl hemoglobin (HbA1c)was determined after the last medication; the ultrastructural changes of kidney were observed by transmission electron microscope; the expressions of collagen Ⅳ (Ⅳ-C), fibronctin (FN), laminin(LN), and the ECM metabolism influencing factors, including MMP-2, tissue inhibitor of metalloproteinase (TIMP-2), transfer growth factor-β1 (TGF-β1) in renal tissue were detected by immunohistological chemistry and image collecting analytical system. Results TSL could decrease the levels of FBG, HbAlc, 24 h mAlb (P<0.05 or P<0.01), ameliorate the thickness of glomerular basement membrane (GBM), decrease the components of ECM, down-regulate TGF-β1 and TIMP-2 expression, and up-regulate MMP-2 expression (P<0.05 or P<0.01). Conclusion TSL plays a role of kidney protection by decreasing the ECM components expression and regulate ECM metabolism. |
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