| 狄柯坪,常立功.红花注射液抑制家兔肠系膜微血管运动的实验研究[J].,2007,(4):339-342 |
| 红花注射液抑制家兔肠系膜微血管运动的实验研究 |
| Experimental Study of Inhibition of Safflower Injection on Mesenteric Microvascular Motion in Rabbits |
| |
| DOI： |
| 中文关键词: 红花注射液 肠系膜 微血管运动 一氧化氮 |
| 英文关键词:Safflower Injection mesentery micro vasomotion nitric oxide |
| 基金项目:国家自然科学基金资助项目(No39070392) |
|
| Hits: 1343 |
| Download times: 2 |
| 中文摘要: |
| 目的在活体情况下实时观察红花注射液(Safflower injection,SI)对家兔肠系膜微血管运动的影响,并探讨一氧化氮(nitric oxide,NO)在其中的作用,以进一步明确红花活血化瘀的作用机理。方法取健康雄性大耳白家兔20只,以乌拉坦腹腔注射行基础麻醉,氯醛酮耳缘静脉注射维持麻醉,气管插管置家兔于自发呼吸状态,同步记录呼吸、血压。在活体情况下运用去甲肾上腺素(noradrenaline,NA)血管运动诱导法诱导出血管运动,分别观察静脉注射NG-单甲基-L-精氨酸(NG-monomethyl-L-arginine,L-NMMA)、SI后血管运动的变化及应用一氧化氮合酶抑制剂L-NMMA对SI作用的影响,摄录结果于监视器上用暂停功能测量。结果单独静脉注射L-NMMA可使被诱导出的血管运动抑制于收缩状态,单独静脉注射SI可使血管运动抑制于舒张状态。在L-NMMA的有效作用时间内应用SI可使被抑制于收缩状态的血管重新扩张,血管运动得以恢复;在SI的有效作用时间内应用L-NMMA则不能使处于舒张状态的血管收缩。结论SI可抑制血管运动于舒张状态并且其作用机制不是通过内源性NO介导的。 |
| 英文摘要: |
| Objective To dynamically observe the effect of Safflower Injection (SI) on mesenteric micro-vascular motion in vivo in rabbits, and to explore the effect of nitric oxide (NO) in the process to further investigate the action mechanism of activating blood to remove stasis of SI. Methods Twenty healthy male Albino were intraperitoneally injected with urethane for basic anesthesia and injected with alpha-chloralose via ear marginal venous to maintain anesthesia, spontaneously ventilated via tracheotomy tube, with the in-step record of breath and blood pressure. The vasomotion was induced by noradrenaline (NA) in vivo, then the changes of vasomotion after injecting SI and NG-monomethyl-L-arginine (L-NMMA, a NO synthase inhibitor) were measured respectively on a TV monitor using a TV camera mounted on the microscope, and the influence of L-NMMA on effect of SI was also observed. Results L-NMMA injection alone can inhibit the NA induced vasomotion in vasoconstriction state, while SI injection alone can inhibit it in vaso-dilation state. SI could abolish the effect of L-NMMA on vasomotion but L-NMMA did not influence the effect of SI on vasomotion. Conclusion SI can inhibit vasomotion in vaso-dilation status, but its mechanism is not mediated by endogenous NO. |
| View Full Text View/Add Comment Download reader |
|
|
|
