| 王少杰,白文,王春玲,戴中.化纤汤治疗博莱霉素致小鼠肺纤维化实验研究[J].,2007,(4):347-351 |
| 化纤汤治疗博莱霉素致小鼠肺纤维化实验研究 |
| Experimental Study on Effect of Huaxian Decoction in Treating Bleomycin-induced Pulmonary Fibrosis in Mice |
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| DOI： |
| 中文关键词: 中药化纤汤 肺纤维化小鼠 博莱霉素 转化生长因子β1 |
| 英文关键词:Huaxian Decoction pulmonary fibrosis mice bleomycin transforming growth factor β1 |
| 基金项目:首都科学发展基金(No2005-SF-Ⅲ-046) |
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| 中文摘要: |
| 目的研究中药化纤汤对博莱霉素致肺纤维化小鼠的防治作用,并与丹参及地塞米松治疗进行对照,从而探讨中药治疗肺纤维化的临床价值及可能的作用机制。方法6~8周龄雄性ICR小鼠分8组,模型组(BM组)经鼻滴入博莱霉素(BLM)5mg/kg建立肺间质纤维化模型,化纤汤预防治疗组(PHT组)于滴入BLM前48h予化纤汤灌胃,化纤汤治疗组(HT组)于滴入BLM后第14天用化纤汤灌胃,同时设立丹参预防治疗组(PDST组)及丹参治疗组(DST组),地塞米松预防治疗组(PDT组)及地塞米松治疗组(DT组),并与正常小鼠(NC组)进行对照。第28天收获其支气管肺泡灌洗液(BALF)和肺组织,右肺行苏木精-伊红(HE)和Masson三色染色,并观察电镜下细胞超微结构的改变,左肺经酸解法检测肺组织羟脯氨酸(HYP)含量,用酶联免疫吸附法(ELISA)检测BALF中转化生长因子β1(TGF-β1)的浓度。结果各药物干预组与BM组比较,小鼠肺系数明显下降(P<0.05);肺泡炎、纤维化程度均有改善(P<0.05),其中化纤汤预防治疗组改善最明显;肺HYP含量下降(P<0.05);BALF中TGF-β1含量亦明显下降。结论早期应用化纤汤可以在某种程度上减轻BLM诱导的肺泡炎及肺纤维化程度,提示益气活血中药对肺纤维化有一定预防作用;抑制TGF-β1在肺组织中的表达可能是其作用机制之一。 |
| 英文摘要: |
| Objective To study the preventive and therapeutic effect of Huaxian Decoction (HXD) on bleomycin-induced pulmonary fibrosis in mice by taking treatment of ligustrazin and prednisone as controls, for investigate the clinical value and possible mechanism in treatment of pulmonary fibrosis by traditional Chinese herbs. Methods A total of 96 male ICR mice, aged 6 to 8 weeks, were randomly divided into 8 groups. Pulmonary fibrosis was induced in the model group by intranasal instillation of bleomycin 5 mg/kg. HXD were given via gastrogavage to the HXD prevention group and treatment group respectively at 48 h before and 14 days after they were modeled with the same method, and the groups of ligustrazin and prednisone, treatment and prevention, were managed the same as for HXD groups, but with ligustazin and prednisone instead of HXD respectively. Bronchoalveolar lavage fluid (BALF) and lung tissue of animals were gathered on day 28. Cellular ultrastructural changes of lung was examined under electron microscope by HE, Masson stain; Content of hydroxyproline (HYP) in lung tissue was assessed by acidolysis method; and concentration of transforming growth factor-β1 (TGF-β1) in BALF was assessed by ELISA. Results Pulmonary coefficient was significantly lower in all the drug intervening groups than that in the model group (P<0.05), improvement on alveolitis and pulmonary fibrosis were also shown after intervention, especially in the HXD prevention group (P<0.05). HYP content in lung tissue and TGF-β1 content in BALF lowered at the same time. Conclusion Early stage application of HXD can alleviate bleomycin-induced alveolitis and pulmonary fibrosis in mice to certain degree, suggesting that Chinese herbs with supplementing qi and activating blood circulation effect could play a definite preventive action on pulmonary fibrosis, and the inhibition on expressions of TGF-β1 in lung tissues may be one of the mechanisms. |
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