| 张伟,张心月,邵雨萌.中医药对各型慢性阻塞性肺疾病大鼠核因子κB和γ-谷氨酰半胱氨酸合酶表达的影响[J].,2007,(5):426-430 |
| 中医药对各型慢性阻塞性肺疾病大鼠核因子κB和γ-谷氨酰半胱氨酸合酶表达的影响 |
| Effects of TCM Treatment According to Syndrome Differentiation on Expressions of Nuclear Factor-κB and γ-Glutamylcysteine Synthetase in Rats with Chronic Obstructive Pulmonary Disease of Various Syndrome Types |
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| DOI： |
| 中文关键词: 慢性阻塞性肺疾病 核因子κB γ-谷氨酰半胱氨酸合酶 中医药 |
| 英文关键词:chronic obstructive pulmonary disease nuclear factor-κB γ-glutamylcysteine synthetase traditional Chinese medicine |
| 基金项目:山东省科技厅资助课题(No.2002山东省科技厅攻关计划第17号) |
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| 中文摘要: |
| 目的通过观察慢性阻塞性肺疾病(COPD)大鼠核因子κB和γ-谷氨酰半胱氨酸合酶(γ-GCS)水平的变化,探讨中医药治疗COPD的机理。方法采用改良烟熏加气管滴加脂多糖方法复制COPD模型,以免疫组化法检测核因子κB和γ-GCS水平。二次造模后,各治疗组分别灌服相应证型的中药合剂,连续14天。寒痰蕴肺治疗组灌服小青龙汤煎剂,痰热阻肺治疗组大鼠灌服麻杏石甘汤煎剂,肺气虚治疗组灌服玉屏风散煎剂,脾气虚治疗组灌服六君子汤煎剂,肾气虚治疗组灌服人参蛤蚧散煎剂,模型组大鼠每天给与2mL生理盐水灌胃。空白对照组不做任何干预。结果与空白对照组相比,各模型组γ-GCS、NF-κB在支气管和肺泡上皮等都为高表达;经过中药治疗后,阳性表达率有明显降低,其差异均有显著性(P<0·05)。结论通过辨证论治各中药方剂能纠正COPD大鼠的氧化/抗氧化失衡,减少炎性反应,从而有效治疗COPD。 |
| 英文摘要: |
| Objective To explore the mechanism of traditional Chinese medicine(TCM) treatment according to syndrome differentiation in treating chronic obstructive pulmonary disease(COPD) by observing the changes of nuclear factor-κB(NF-κB) and γ-glutamylcysteine synthetase(γ-GCS) expression levels in rats.Methods COPD model was established by modified method of combining fumigation and lipopolysaccharide(LPS) intra-tracheal dripping.Model rats were treated respectively for succesive 14 days according to their syndrome,that is,Xiaoqinglong Decoction to the rats of cold-phlegm accumulation in Fei,Maxing Shigan Decoction to those of heat-phlegm accumulation in Fei,Yupingfeng Decoction to those of Fei-qi deficiency,Liujunzi Decoction to those of Pi-qi deficiency,Renshen Gejie Decoction to those of Shen qi-deficiency.Besides,model rats in the model control group received 2mL normal saline daily,and no intervention was applied in the normal control group.The expression of γ-GCS and NF-κB was detected by immunochemistry before and after treatment.Results Compared with that in the normal rats,the expressions of γ-GCS and NF-κB in bronchial and alveolar epithelium of COPD rats before treatment were significantly higher,but the positive expression rates were lowered after treatment significantly(P<0.05).Conclusion TCM treatment according to syndrome differentiation could rectify imbalance of oxidation/anti-oxidation and alleviate inflammatory reaction in COPD rats,thus to treat COPD effectively. |
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