| 张海啸,史载祥,贾海忠,李格,程文立,谷万里,杨仲义.大蒜素对NIH3T3细胞增殖及胶原合成的影响[J].,2007,(5):431-434 |
| 大蒜素对NIH3T3细胞增殖及胶原合成的影响 |
| Effects of Garlicin on NIH3T3 Cell Proliferation and Collagen Synthesis |
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| DOI： |
| 中文关键词: 大蒜素 NIH3T3细胞 Ⅰ型胶原 细胞增殖 羟脯氨酸 |
| 英文关键词:garlicin NIH3T3 cell type Ⅰ collagen cell proliferation hydroxyproline |
| 基金项目:首都医学发展科研基金的资助课题(No.03-Ⅲ-02) |
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| 中文摘要: |
| 目的研究大蒜素对NIH3T3细胞生长增殖和Ⅰ型胶原合成的影响,探讨其抗纤维化作用机理。方法大蒜素加入NIH3T3细胞培养液,以丹参为对照药物,测定H3-thymidine DNA掺入量;碱消化法检测细胞培养液中羟脯氨酸含量,荧光免疫染色方法检测NIH3T3细胞Ⅰ型胶原蛋白的表达。结果大蒜素在0·2-5μg/mL剂量范围内剂量依赖的抑制NIH3T3细胞生长和增殖;药物作用后,细胞培养液中的羟脯氨酸含量减少,Ⅰ型胶原蛋白的表达减少。结论大蒜素对NIH3T3细胞生长增殖有抑制作用,减少细胞胶原的合成,减少Ⅰ型胶原的表达,可能具有抗纤维化作用。 |
| 英文摘要: |
| Objective To investigate the effects of garlicin on fibroblasts proliferation and type Ⅰ collagen synthesis and explore its anti-fibrosis mechanism.Methods Garlicin was added into the culture fluid of NIH3T3 cell,taking Radix Salviae miltiorrhizae as the control medicine.The spiking of H3-thymidine DNA was detected,also the hydroxyproline(HOP) concentration in the culture fluid by alkali digestion method and the protein expression of typeⅠ collagen in NIH3T3 cells by immunofluorescent staining.Results The NIH3T3 cell growth and proliferation rate were obviously reduced after garlicin treatment concentration-dependently in range of 0.2-5 μg/mL;HOP level and protein expression of type Ⅰ collagen also lowered.Conclusion Garlicin could inhibit NIH3T3 cell proliferation,reduce the synthesis and protein expression of type Ⅰ collagen so as to exert the anti-fibrosis effect. |
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