| 刘健,杨梅云,范海霞.佐剂关节炎大鼠行为、脑组织氨基酸的变化及新风胶囊对其的影响[J].,2007,(5):444-448 |
| 佐剂关节炎大鼠行为、脑组织氨基酸的变化及新风胶囊对其的影响 |
| Changes of Behavior and Brain Amino Acids of Rats with Adjuvant Arthritis and Effects of Xinfeng Capsule on Them |
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| DOI： |
| 中文关键词: 佐剂关节炎 大鼠行为 脑组织氨基酸 新风胶囊 |
| 英文关键词:adjuvant arthritis rat’s behavior amino acids in brain tissue Xinfeng Capsule |
| 基金项目:国家中医药管理局科研课题专项基金(No.04-05LP27);安徽省自然科学基金项目(No.050431002) |
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| 中文摘要: |
| 目的观察佐剂关节炎(adjuvant arthritis,AA)大鼠行为、脑组织氨基酸的变化及新风胶囊(XFC)对其的影响。方法用弗氏完全佐剂(complete Freund’s adjuvant,CFA)诱发大鼠产生关节炎,设立正常对照组、模型对照组、XFC治疗组、甲氨喋呤(MTX)治疗组、雷公藤多苷片(TPT)治疗组。观察AA大鼠行为、脑组织氨基酸的变化和关节肿胀度、关节炎指数(arthritis index,AI)的变化以及XFC对其的影响,并进行相关性分析。结果与正常对照组比较,模型对照组大鼠自主活动次数明显减少、跳台错误次数均明显增多、大鼠跳台的时间(step down latency,SDL)缩短而反应时间(escape latency,EL)延长,脑组织中γ-氨基丁酸(GABA)含量明显升高,而谷氨酸(GLU)/GABA值明显降低(P<0·05)。与模型对照组比较,XFC组大鼠的自主活动次数显著增加、SDL延长、EL缩短、跳台错误次数减少,GABA明显下调、GLU/GABA上调(P<0·05),而MTX、TPT组改善不明显(P>0·05)。各治疗组大鼠的肿胀度、关节炎指数均明显降低(P<0·05);AA大鼠的自主活动次数、SDL与足跖肿胀度、关节炎指数、甘氨酸(GLY)、GABA呈负相关,与GLU/GABA值呈正相关(P<0·05);EL、跳台错误次数均与关节炎指数、GLY、GABA呈正相关,与GLU/GABA值呈负相关(P<0·05)。结论免疫应激可引起大鼠行为改变及脑组织氨基酸的变化,XFC可通过调节脑组织氨基酸递质改善AA大鼠行为。 |
| 英文摘要: |
| Objective To observe the changes of behavior and amino acids in brain tissue of rats with adjuvant arthritis,and effects of Xinfeng Capsule(XFC) on them.Methods AA model was induced by complete Freund’s adjuvant in rats.Five groups including the normal control group,the model group,the XFC group,the methotrexate(MTX) group and the Tripterygium wilfordii polysaccharide(TPT) group were set up.The changes of behavior,amino acids in brain tissue,swelling degree of joints,and arthritis index(AI) were observed and the correlation analysis on these indexes was performed.Results Compared with those in the normal control,the frequency of independent activity obviously decreased in the model rats,accompanied with increased number of step-down errors,shortened step down latency(SDL) and prolonged escape latency(EL),also the level of γ-aminobutyric acid(GABA) in brain tissue elevated and glutamic acid(GLU)/GABA reduced(P<0.05).Compared with those in the model group,all the above indexes were improved significantly in the XFC group after treatment(P<0.05),while they changed insignificantly in the MTX and TPT groups(P>0.05),the degree of joint swelling and AI were obviously lower in the 3 treated groups(P<0.05).Correlation analysis showed the frequency of independent activity and SDL were negatively correlated to the swelling degree of joint,and levels of AI,glycine(GLY) and GABA respectively,but positively correlated with GLU/GABA(P<0.05);while EL and number of step-down errors were positively correlated to AI,GLY and GABA respectively,but negatively correlated to GLU/GABA(P<0.05).Conclusion Immune stress could lead to the changes of rat behavior and amino acids in brain tissue.XFC treatment could improve the behavior of AA rats through regulating the levels of amino acids in brain tissue. |
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