| 陈忻,张楠,邹海艳,赵晖,穆阳.黄芩苷对MPTP帕金森病小鼠的保护作用[J].,2007,(11):1010-1012 |
| 黄芩苷对MPTP帕金森病小鼠的保护作用 |
| Protective Effect of Baicalin on Mouse with Parkinson’s Disease Induced by MPTP |
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| DOI： |
| 中文关键词: 黄芩苷 帕金森病 1-甲基-4-苯基-1,2,3,6-四氢吡啶 |
| 英文关键词:Baicalin Parkinson’s disease 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine |
| 基金项目:北京市教委科技发展计划资助项目(No.KM200610025008) |
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| 中文摘要: |
| 目的观察黄芩苷对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)帕金森病小鼠的保护作用。方法以10月龄(老龄)C57BL小鼠腹腔注射MPTP(每天30mg/kg)3天制备小鼠帕金森病模型;黄芩苷组灌胃黄芩苷(每天100mg/kg,共15天);用悬挂、游泳实验检测小鼠肢体运动功能;HPLC法测定小鼠脑纹状体多巴胺(DA)含量;分光光度法测定小鼠脑组织谷胱苷肽(GSH)、谷胱苷肽过氧化酶(GSH-Px)及丙二醛(MDA)的含量;小鼠脑冰冻切片,行酪氨酸羟化酶(TH)免疫组化染色,观察中脑黑质多巴胺能神经损伤情况。结果(1)模型组小鼠悬挂和游泳实验分值及脑纹状体DA含量均显著降低,黑质TH阳性细胞显著丢失,说明模型复制成功。(2)黄芩苷能够防止帕金森病小鼠黑质多巴胺能神经细胞丢失,防止模型小鼠纹状体DA含量的降低,并显著升高小鼠脑内GSH水平。但短时间给黄芩苷不能明显改善MPTP致帕金森病小鼠运动功能障碍。结论黄芩苷预防给药对MPTP致帕金森病小鼠有保护作用。 |
| 英文摘要: |
| Objective To observe the protective effect of baicalin on mouse with Parkinson’s disease induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP). Methods The mouse model of Parkinson’s disease was established by intraperitoneal injection of MPTP at the daily dose of 30 mg/kg for 3 days to the aged (ten months old) C57BL mouse. And to the model mice in the tested group, baicalin (100 mg/kg) was given via gastric perfusion per day for 15 days. The motor function of limbs in mice was tested through hanging and swimming tests; the dopamine content of striatum was measured by HPLC; and the contents of malondialdehyde (MDA), glutathione (GSH) and glutathione peroxidase (GSH-Px) in the brain tissue were measured by spectrophotography. Besides, the freezing section of mouse brain was made through immunohistochemical stain with tyrosine hydroxylase (TH) to determine the condition of dopaminergic neuron damage in mesencephalon. Results (1) The decreased score in the hanging test and swimming test, the reduced DA contents of striatum and lessening of TH positive neurons in substantial nigra illustrated the model of Parkinson’s disease was successfully established. (2) Medication of baicalin could prevent the loss of TH positive neurons in substantial nigra and the decrease of dopamine content of striatum in Parkinson mouse, and significantly raise the content of GSH in the brain, but MPTP induced motor dysfunction in model mouse was not significantly improved by a short-time medication. Conclusion The preventive medication of baicalin shows a protective effect on C57BL mouse with Parkinson’s disease induced by MPTP. |
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