| 张燕萍,樊茂蓉,王书臣,苗青,王伟,张元元,林家扬.肺纤平对博莱霉素所致肺纤维化大鼠Ⅰ、Ⅲ型胶原的影响[J].,2007,(11):1013-1015 |
| 肺纤平对博莱霉素所致肺纤维化大鼠Ⅰ、Ⅲ型胶原的影响 |
| Effects of Feixianping on Collagen Type Ⅰ and Ⅲ in Bleomycin Induced Pulmonary Fibrosis Rats |
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| DOI： |
| 中文关键词: 肺纤平 肺纤维化 Ⅰ、Ⅲ型胶原 免疫组织化学 |
| 英文关键词:Feixianping pulmonary fibrosis collagen typeⅠ and Ⅲ immunohistochemistry |
| 基金项目:国家自然科学基金资助项目(No.30340076) |
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| 中文摘要: |
| 目的观察肺纤平对肺纤维化大鼠Ⅰ、Ⅲ型胶原的影响。方法将60只健康雄性SD大鼠随机分为5组,即正常组、模型组、阳性药(泼尼松)组和肺纤平低、高剂量组。除正常组外,其余4组用博莱霉素注入大鼠气管内制作肺纤维化模型,各组于造模后第1天给予相应药物,分别于第7、14、28天处死大鼠,取肺组织进行HE染色,普通光镜下观察大鼠肺组织病理学变化,并进行免疫组化染色,观察大鼠肺组织Ⅰ、Ⅲ型胶原的变化。结果HE染色显示,造模后第14天开始大鼠肺组织肺泡间壁内出现淡染、增生的胶原纤维,肺泡间壁略有增厚,第28天较第14天时病变更加明显;模型组胶原纤维增生最多,各治疗组病变较模型组轻,且Ⅰ、Ⅲ型胶原增生减少(P<0.05),肺纤平高剂量组给药14天Ⅰ、Ⅲ型胶原增生减少,其减少程度优于阳性药组(P<0.05)。结论肺纤平可以有效地抑制实验性肺纤维化大鼠胶原的异常增生。 |
| 英文摘要: |
| Objective To study the effect of Feixianping (FXP) on collagen typeⅠand Ⅲ in rats with pulmonary fibrosis (PF). Methods Sixty healthy male SD rats were randomly divided into 5 groups, the normal group (A), the model group (B), the positive control group (C) and the two FXP groups (D and E) treated respectively with high and low dose of FXP. Except those in Group A (they were not modeled and administered with normal saline), all rats were established into PF model by intra-tracheal instillation of bleomycin and administered with respective medicines starting from the 1st day after modeling. Rats were sacrificed in batches at 3 time points, the 7th, 14th, and 28th day for observing the pathological changes of lung under light microscope with HE staining and to identify collagen typeⅠ and Ⅲ in lung tissue by immunohistochemical stain and image quantitative analysis. Results Light-dyeing proliferative collagen fiber was presented in the slightly thickened alveolar wall in lung of modeled rats from the 14th day on, and the pathological changes became more distinct on the 28th day. The highest amount of collagen appeared in the group B, correspondingly, that in all the other groups was much lower (P<0.05). Reduction of collagen typeⅠ and Ⅲ revealed in both FXP treated groups, but better effect was shown in the high dose FXP group. The effect of FXP was superior to that of positive control on the 14 th day (P<0.05). Conclusion FXP can effectively reduce the abnormal proliferation of collagen in experimental rats with PF. |
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