| 张京春,陈可冀,刘剑刚,张文高,史大卓,刘龙涛,殷惠军,徐浩.解毒活血配伍方药对载脂蛋白E基因敲除小鼠血清超敏C反应蛋白的影响[J].,2008,(4):330-333 |
| 解毒活血配伍方药对载脂蛋白E基因敲除小鼠血清超敏C反应蛋白的影响 |
| Effect of Assorted Use of Chinese Drugs for Detoxifying and Activating Blood Circulation on Serum High Sensitive C-reactive Protein in Apolipoprotein E Gene Knock-out Mice |
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| DOI： |
| 中文关键词: 解毒活血配伍方药 载脂蛋白E基因敲除小鼠 超敏C反应蛋白 动脉粥样硬化 易损斑块 |
| 英文关键词:assorted use of Chinese drugs for detoxifying and activating blood circulation apolipoprotein E gene knock-out mice high sensitive C-reactive protein atherosclerosis vulnerable plaque |
| 基金项目:国家重点基础研究发展计划(973计划)项目(No.2006CB504803);国家中医药管理局科技项目基金课题(No.0405JP64) |
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| 中文摘要: |
| 目的观察解毒活血配伍方药对载脂蛋白E基因敲除[apolipoprotein E gene knock-out,ApoE(-/-)mice]小鼠血清超敏C反应蛋白(hs-CRP)的影响。方法13周龄110只ApoE(-/-)小鼠分为高脂饲料组(98只,给予高脂饲料),普通饲料组(12只,给予普通饲料),同时设13周龄C57BL/6J小鼠作为正常对照组(12只,给予普通饲料)。19周后随机抽取高脂饲料ApoE(-/-)小鼠2只确认易损斑块形成后,剩余96只随机分为8组(模型组、解毒组、活血组、解毒活血配伍高、中、低剂量组、洛伐他汀组和血脂康组),每组12只。造模成功后每天给予药物干预,解毒组予虎杖提取物26·6mg/kg;活血组予芎芍胶囊110mg/kg;配伍高剂量组予虎杖提取物53·2mg/kg,芎芍胶囊220mg/kg;配伍中剂量组给予虎杖提取物26·6mg/kg,芎芍胶囊110mg/kg;配伍低剂量组给予虎杖提取物13·3mg/kg,芎芍胶囊55mg/kg;洛伐他汀组给予洛伐他汀3·3mg/kg;血脂康组给予血脂康0·2g/kg;以上药物根据剂量蒸馏水溶解,混匀后灌胃,每次0·4mL。模型组、普通饲料组、C57BL/6J小鼠对照组均灌服生理盐水0·4mL。用药17周后,下腔静脉取血,全自动酶标仪检测血清hs-CRP浓度。结果模型组血清hs-CRP水平显著高于正常对照组和普饲组(P<0·05,P<0·01);各给药组中,洛伐他汀组、解毒组和配伍高剂量组hs-CRP水平下降,与模型组比较,差异有统计学意义(P<0·01);配伍高剂量组hs-CRP水平低于洛伐他汀组、血脂康组、单纯解毒或活血组及配伍中、低剂量组;解毒组hs-CRP水平低于活血组(P<0·01)。结论解毒活血配伍方药可降低ApoE(-/-)小鼠血清hs-CRP水平。 |
| 英文摘要: |
| ObjectiveTo observe the regulatory effect of assorted use of Chinese drugs for detoxifying and activating blood circulation on serum high sensitive C-reactive protein (hs-CRP) in apolipoprotein E gene knock-out 〔ApoE(-/-)〕mice. MethodsApoE (-/-) mice of 13-week old were devided into two groups, 12 in Group A fed with common forage and 98 in Group B with high lipid forage. Besides, 12 C57 BL/6J mice, 13-week old, were set as Group C fed with common forage. After being fed for 19 weeks, and the formation of vulnerable plaque had been confirmed in 2 mice of Group B, the other 96 mice were sub-divided into 8 groups, 12 in each group. Except that 0.4 mL normal saline was infused to the Group B1 as well as Group A and C, the other 7 sub-groups of Group B were respectively medicated with various drugs as follows: B2, Polydatin (PD, an extract from giant knotweed rhizome for detoxicating) 26.6 mg/kg; B3, Xiongshao Capsule (XS, a Chinese herbal preparation for activating blood circulation), 110 mg/kg; B4, PD 53.2 mg/kg+XS 220 mg/kg; B5, PD 26.6 mg/kg+XS 110 mg/kg; B6, PD 13.3 mg/kg+XS 55 mg/kg; B7, Lovastatin 3.3 mg/kg; and B8, Xuezhikang (XZK, a Chinese patent drug) 0.2 g/kg, all were administered by dissolving in 0.4 mL of distilled water for gastrogavage. The serum contents of hs-CRP were detected, with blood sample drawing from inferior vena cava, using enzyme-linked immunosorbent assay 17 weeks after medication. ResultsThe hs-CRP level was significantly higher in Group B1 than in Groups A and C (P<0.05,P<0.01). Comparisons between various sub-groups of Group B in hs-CRP content showed that hs-CRP in Group B2, B4 and B7 was lower than that in B1 (P<0.01), hs-CRP in Group B4 was the lowest, and hs-CRP was lower in Group B2 than in Group B3. ConclusionAssorted use of Chinese drugs for detoxifying and activating blood circulation could reduce serum hs-CRP level in ApoE(-/-) mice. |
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