| 朱培成,禤国维,陈达灿,范瑞强.原花青素对斑秃患者外周血单核细胞中IFN-γ、IL-12及转录因子T-bet基因表达的调节作用[J].,2008,(10):900-902 |
| 原花青素对斑秃患者外周血单核细胞中IFN-γ、IL-12及转录因子T-bet基因表达的调节作用 |
| Regulatory Effect of Procyanidins on the Expressions of Interferon-γ,Interleukin-12 and Transcription Factor T-bet mRNA in Peripheral Blood Mononuclear Cell of Patients with Alopecia Areata |
| |
| DOI： |
| 中文关键词: 斑秃 细胞因子 T-betmRNA 基因表达 原花青素 |
| 英文关键词:alopecia areata cytokine T-bet mRNA gene expression procyanidins |
| 基金项目:广东省科技厅资助课题(No.2002c3110502) |
|
| Hits: 1555 |
| Download times: 1 |
| 中文摘要: |
| 目的探讨原花青素对斑秃患者外周血单核细胞(PBMC)中Th1型细胞因子(IFN-γ、IL-12)及转录因子T-bet基因表达的调节作用。方法用逆转录-聚合酶链反应(RT-PCR)法,分别检测8例轻型斑秃、12例重型斑秃患者及10例健康人外周血PBMC经原花青素(PC)刺激后IFN-γ、IL-12及T-bet mRNA的表达状况。结果植物血凝素(phytoaemagglutinin,PHA)及PC+PHA共同刺激重型斑秃患者PBMC后,转录因子T-bet mRNA分别为0.581±0.148、0.419±0.113;IFN-γ mRNA分别为0.689±0.219、0.430±0.162;IL-12 mRNA分别为0.198±0.056、0.136±0.035;与自身对照比较,差异均有统计学意义(P<0.05)。结论PC可抑制重型斑秃患者PBMC中转录因子T-bet及Th1型细胞因子基因表达,逆转Th1型反应,含PC的中药松针治疗斑秃有效的机制可能部分与此相关。 |
| 英文摘要: |
| Objective To investigate the regulatory effect of procyanidins(PC)on the expressions of Th1 type cytokines(IFN-γ and IL-12)and the transcription factor T-bet mRNA in peripheral blood mononuclear cell(PBMC)in patients with alopecia areata(AA).Methods Above-mentioned expressions were detected using RT-PCR technique in 20 AA patients(8 of mild and 12 of severe degree)and 10 healthy subjects after phytoaemagglutinin(PHA)or PHA+PC stimulation.Results After being stimulated by PHA and PHA+PC,in patients with severe AA,the expression of T-bet mRNA was 0.581±0.148 and 0.419±0.113 respectively;that of IFN-γ mRNA,0.689±0.219 and 0.430±0.162;and that of IL-12 mRNA,0.198±0.056 and 0.136±0.035,respectively.As compared with those before stimulation,the respective difference was statistically significant(P<0.05).Conclusions PC can inhibit the expressions of Th1 type cytokines and transcription factor T-bet in PBMC of severe AA patients,and reverse the Th1 responses.The authors offered that it maybe part of the acting mechanism of pine needle for treatment of AA. |
| View Full Text View/Add Comment Download reader |
