| 谭俊珍,李庆雯,南亚昀,范英昌.丹酚酸B预处理内皮祖细胞在大鼠骨髓间充质干细胞分化过程中对心肌早期基因表达的影响[J].,2009,(6):529-532 |
| 丹酚酸B预处理内皮祖细胞在大鼠骨髓间充质干细胞分化过程中对心肌早期基因表达的影响 |
| Effects of Salvianolic Acid B Preconditioning Endothelial Progenitor Cells on Expressions of Myocardial Genes in Bone Mesenchymal Stem Cells at the Early Cell Differentiation Stage of Rats |
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| DOI： |
| 中文关键词: 急性心肌梗死 内皮祖细胞 骨髓间充质干细胞 丹酚酸B 细胞移植 |
| 英文关键词:acute myocardial infarction endothelial progenitor cells bone mesenchymal stem cells salvianolic acid B cell transplantation |
| 基金项目:国家自然科学基金资助(No.30572443) |
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| 中文摘要: |
| 目的探讨丹酚酸B预处理内皮祖细胞(EPCs)联合大鼠骨髓间充质干细胞(BMSc)移植于梗死心肌对BMSc分化过程中早期基因Nkx2.5、GATA-4 mRNA表达的影响,从而找出能够使两种细胞达到最佳协同作用的移植方法。方法分离培养大鼠BMSc、EPCs,将Wistar大鼠冠状动脉左前降支结扎制成急性心肌梗死模型后,在梗死心肌处植入BMSc和丹酚酸B预处理的不同浓度EPCs或DMEM培养液。移植后4周,实时荧光定量PCR检测丹酚酸B预处理的EPCs在BMSc分化过程中对心肌早期基因Nkx2.5和GATA-4 mRNA表达的影响。结果与模型组比较,各细胞移植组Nkx2.5及GATA-4 mRNA表达均明显增加(P<0.01,P<0.05);各细胞移植组之间,EPCs含量高(EPCs∶BMSc为8∶1组)的移植组Nkx2.5(2.654±0.606)、GATA-4 mRNA(1.573±0.372)表达增加更明显。结论BMSc在心肌微环境可向心肌样细胞分化,其分化情况与其内环境改善程度有关。 |
| 英文摘要: |
| Objective To investigate the effects of Salvianolic acid B preconditioned endothelial progenitor cells (EPCs) on the Nkx2.5 and GATA-4 gene expressions at the early stage of cell differentiation of bone mesenchymal stem cells (BMSc) transplanted into infarcted myocardium,in order to find out the best synergism for co-transplantation of the two kinds of cells. Methods BMSc and EPCs of rats were isolated and cultured,and rats were modeled into acute myocardial infarction (AMI) by left coronary artery ligation. Then the EPCs preconditioned with different concentrations of Salvianolic acid B and BMSc or DMEM medium were implanted into heart ischemia area. Expressions of Nkx2.5 and GATA-4 mRNA expressions in myocardium were detected by Real-time RT-PCR 4 weeks later. Results Compared with those in the non-implanted model rats′ myocardium,the gene expression of Nkx2.5 and GATA-4 mRNA were significantly higher in all the transplantation receptive groups,comparisons between the implanted groups showed that the highest value of expressions (2.654±0.606 of Nkx2.5 and 1.573±0.372 of GATA-4) displayed in the group contained more EPCs,for 8-fold to BMSc in volume. Conclusion BMSc can differentiate into cardiac muscle like cells,and condition of their differentiation is related with the degree of the internal environment improved. |
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