| 高磊,李威,匡洪影,侯丽辉,吴效科.小檗碱和葛根素对地塞米松诱导卵泡膜细胞胰岛素抵抗的影响[J].,2009,(7):623-627 |
| 小檗碱和葛根素对地塞米松诱导卵泡膜细胞胰岛素抵抗的影响 |
| Effects of Berberine and Puerarin on Dexamethasone-Induced Insulin Resistance in Porcine Ovarian Thecal Cells |
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| DOI： |
| 中文关键词: 胰岛素抵抗 卵泡膜细胞 小檗碱 葛根素 多囊卵巢综合征 |
| 英文关键词:insulin resistance thecal cell berberine puerarin polycystic ovary syndrome |
| 基金项目:中国-芬兰科学技术合作基金(2004-2006第11次会议);国家自然科学基金资助项目(No.30572404);黑龙江省自然科学基金重点项目(No.ZJY0506-01) |
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| 中文摘要: |
| 目的探索地塞米松人工诱导猪卵泡膜细胞产生胰岛素抵抗,以及中药小檗碱和葛根素对胰岛素抵抗的卵泡膜细胞功能的调控作用。方法收集新鲜猪卵泡膜细胞进行体外培养,经地塞米松作用48h后,检测猪卵泡膜细胞对糖利用率情况。同时采用逆转录-聚合酶链反应(RT-PCR)、蛋白质免疫印迹技术(Westblotting),检测小檗碱和葛根素对卵泡膜细胞内胰岛素信号传导分子和甾体激素合成的影响,采用化学发光法测定激素水平。结果(1)地塞米松作用48h后细胞内[3-3H]标记的葡萄糖摄取量降低约1/2;细胞上清液葡萄糖含量升高约1/3。(2)RT-PCR和Westblotting结果显示:卵泡膜细胞产生胰岛素抵抗后,胰岛素受体底物-1(IRS-1)、葡萄糖转运蛋白4(GLUT4)的mRNA和蛋白表达水平下降;过氧化酶活化增生受体(PPAR)γ和细胞色素P45017羟化酶(CYP17)的mRNA或蛋白表达水平上升。应用中药小檗碱和葛根素进一步作用48h后,抵抗细胞内的上述胰岛素信号分子和CYP17表达明显逆转。(3)猪卵泡膜细胞产生胰岛素抵抗后,睾酮(T,mg/L)水平模型组(0.82±0.20)较对照组(0.38±0.01)升高(P<0.05),小檗碱组(0.44±0.24)和葛根素组(0.45±0.21)比模型组降低(P<0.05)。血清孕酮(P)水平各组未见明显变化(P>0.05)。结论卵泡膜细胞产生胰岛素抵抗后雄激素合成能力显著增强,中药小檗碱和葛根素能降低卵泡膜细胞的胰岛素抵抗程度和雄激素合成,提示中药增敏剂对多囊卵巢综合征的治疗有开发前景。 |
| 英文摘要: |
| Objective To explore the direct contribution of dexamethasone(Dex)for insulin resistance inducing in thecal cells and effects of berberine(Ber)and puerarin(Pue).Methods Ovarian thecal cells from porcine follicles were isolated and cultured in vitro.Insulin resistance of thecal cells was induced by Dex treatment for 48 h.Then the glucose utilization ratio of thecal cells was detected.Meanwhile,the effects of Ber and Pue on insulin signal transmission and steroid hormones synthesis were determined by RT-PCR and Western blotting.Results(1)After being treated by Dex for 48 h,the [3-3H ]-glucose uptake in cells was lowered by about half,and the glucose content in supernate increased for about 1/3.(2)The RT-PCR and Western blotting showed that levels of insulin receptor substrate-1(IRS-1),mRNA and protein expression of glucose transporter 4(GLUT4)lowered,peroxisome proliferator-activated receptor-γ(PPARγ)and cytochrome P450 17 hydroxylase(CYP17)mRNA or protein expression increased in the model cells.However,the changes of above insulin signal molecules and CYP17 expression were inversed significantly after treated with Ber and Pue for 48 h.(3)As compared with the control,in the model cells,levels of testosterone(T,μg/mL)was higher(0.82±0.20 vs 0.38±0.01,P<0.05),while after Ber and Pue treatment it was 0.44±0.24 and 0.45± 0.21 respectively,all lower than that in the model cells(P<0.05).No significant change of serum progesterone was found in all groups(P>0.05).Conclusions After insulin resistance has been induced,the androgen synthetic capacity of thecal cells enhanced significantly.Ber and Pue could lower the degree of insulin resistance and the androgen synthesis in the model cells,displaying the favorable prospect of the two insulin sensitizing agents for the treatment of polycystic syndrome. |
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