| 林胜璋,荆河,杨潇.大黄素体外抑制人树突状细胞分化及成熟作用的研究[J].,2009,(9):806-809 |
| 大黄素体外抑制人树突状细胞分化及成熟作用的研究 |
| Inhibitory Effect of Emodin on the Differentiation and Maturation of Dendritic Cells in vitro |
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| DOI： |
| 中文关键词: 大黄素 细胞毒T细胞 树突状细胞 |
| 英文关键词:emodin cell toxicant T-lymphocyte dendritic cell |
| 基金项目:国家自然科学基金资助项目(No.30572395) |
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| 中文摘要: |
| 目的探讨大黄素体外作用树突状细胞(dendritic cell,DC)后对人DC分化、成熟及免疫功能的影响。方法分离健康人外周血单个核细胞,在粒-巨噬细胞集落刺激因子(GM-CSF)和白细胞介素-4(IL-4)的诱导下培养DC。培养的第5天和第7天分别加入脂多糖(liopolysaccharide,LPS)及大黄素刺激获得成熟或未成熟DC,流式细胞仪检测树突状细胞表型(HLA-DR、CD80、CD86、CD83、CD14、CD11c)及IL-12的分泌水平,并将DC与自身T淋巴细胞共培养,评价树突状细胞的免疫刺激功能。结果大黄素作用组DC细胞表面分子CD80及CD83的表达分别为(13.4±6.6)%和(9.3±2.2)%,而对照组分别为(39.3±8.6)%和(30.7±5.6)%,差异有统计学意义(P<0.05);大黄素组与对照组比较,DC分泌IL-12[(1 700.44±1 000.21)μg/Lvs(4 500.60±1 200.60)μg/L]能力下降,分泌IL-10能力提高[(350.60±150.20)μg/Lvs(230.70±90.10)μg/L]差异均有统计学意义(P<0.05)。混合淋巴细胞培养检测发现大黄素可显著抑制DC刺激的自体T淋巴细胞的增殖。结论在DC体外转化的过程中大黄素可明显抑制DC的成熟及免疫刺激功能。 |
| 英文摘要: |
| Objective To study the effect of emodin on the differentiation,maturation and function of human dendritic cells(DC) in vitro.Methods Cells isolated from human peripheral blood mononuclear cells(PBMCs) were induced to dendritic cells(DC) with recombinant interleukin-4 and recombinant granulocyte-macrophage colony stimulating factor(GM-CSF).Liopolysaccharide(LPS) and different concentrations of emodin were added respectively in the cultured cells on the 5th and the 7th to obtain mature or immature DCs.The phenotype of DCs(HLA-DR,CD80,CD86,CD83,CD14,CD11c) and the secretion of interleukin-12(IL-12) were analyzed by flow cytometry,and the immuno-stimulating function of DCs was evaluated by co-culture of DCs and self-T-lymphocytes.Results The expression rate of CD80 and CD83 in the emodin group were 13.4%±6.6% and 9.3%±2.2% respectively;which were significantly lower than those in the control group(39.3%±8.6% and 30.7%±5.6%),respectively(P<0.05).IL-12 secretion of DCs was lower(1 700.44±1 000.21 μg/L vs 4 500.60±1 200.6 μg/L) but IL-10 secretion was higher(350.6±150.2 μg/L vs 230.7±90.1 μg/L) in the emodin group than in the control group(P<0.05).Mixed lymphocyte cultuke(MLR) examination showed that emodin could significantly inhibit the stimulation of DCs on self-T-lymphocyte proliferation.Conclusion Emodin could evidently suppress the maturation and immune stimulating function of DCs during their in vitro conversion process. |
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