| 张海涛,杨跃进,程宇彤,康晟,赵京林,孟亮,田毅,张燕婉,叶珏.通心络预给药2h对猪急性心肌梗死再灌注后心肌无再流和细胞因子变化的影响[J].,2009,(9):821-824 |
| 通心络预给药2h对猪急性心肌梗死再灌注后心肌无再流和细胞因子变化的影响 |
| Effect of Tongxinluo on Mini-swine Cytokines and Myocardial No-reflow in Early Reperfusion of Acute Myocardial Infarction |
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| DOI： |
| 中文关键词: 急性心肌梗死 无再流 细胞因子 心肌声学造影 通心络 |
| 英文关键词:acute myocardial infarction no-reflow cytokine myocardial contrast echocardiography Tongxinluo |
| 基金项目:国家重点科技研究发展计划(973计划)资助课题(No.2005CB523303) |
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| 中文摘要: |
| 目的评价急性心肌梗死(AMI)猪缺血再灌注前2 h给予通心络对血清细胞因子水平和心肌无再流变化的干预效果。方法中华小型猪40只,随机分成假手术组、AMI对照组、通心络小剂量(0.1 g/kg)、中剂量(0.2 g/kg)和大剂量(0.4 g/kg)组,每组各8只。冠状动脉前降支阻断1.5 h,再灌注3 h建立AMI再灌注动物模型。各通心络治疗组于AMI缺血再灌注前2 h行灌胃给药。测定并比较各组AMI前、AMI 1.5 h、再灌注后3 h血清细胞因子水平变化。于AMI 1.5 h和再灌注3 h行心肌声学造影(myocardial contrast echocardiography,MCE),测定并比较心肌无再流面积变化。结果(1)与对照组比较,仅大剂量通心络组在AMI 1.5 h P-选择素(P-selectin)、细胞间黏附分子-1(ICAM-1)水平显著降低(均P<0.05),而再灌注后3 h P-选择素、细胞间黏附分子-1、血管细胞黏附分子-1(VCAM-1)、白细胞介素-6(IL-6)水平均显著降低(均P<0.05),白细胞介素-10(IL-10)水平显著升高(P<0.05);(2)与对照组比较,大剂量通心络能够显著缩小再灌注后3 h心肌无再流区面积[分别为(6.59±1.73)cm2、(4.68±1.53)cm2,P<0.05]。中小剂量组则差异无统计学意义。结论在AMI猪缺血再灌注前2 h予大剂量通心络可显著降低再灌注后细胞黏附和促炎症因子水平,调高抗炎症因子水平,并缩小心肌无再流面积,为临床应用提供了实验基础。 |
| 英文摘要: |
| Objective To assess the effect of Tongxinluo on cytokines and myocardial no-reflow in early reperfusion of acute myocardial infarction(AMI).Methods Forty mini-swine were divided into five groups randomly,sham group,control group,low dose(0.1 g/kg),medium dose(0.2 g/kg) and high dose(0.4 g/kg) group of Tongxinluo(which were administered 2 h before reperfusion),eight swine in each group.Animals except those in the sham group were subjected to 1.5 h of coronary occlusion followed by 3 h of reperfusion.Serum contents of P-selectin,intercellular adhesion molecule 1(ICAM-1),vascular cell adhesion molecule 1(VCAM-1),interleukin 6(IL-6) and interleukin 10(IL-10),as well as myocardial contrast echocardiography(MCE) were evaluated at baseline,and after 1.5 h of AMI and 3 h of reperfusion.Results(1) Compared with that of the control group,high dose of Tongxinluo could reduce serum contents of P-selectin and ICAM-1 at 1.5 h of AMI(all P<0.05),and P-selectin,ICAM-1,VCAM-1,and IL-6 at 3 h of reperfusion significantly(all P<0.05),accompanied by significantly elevated IL-10(P<0.05).(2) Compared with that of control group,high dose of Tongxinluo could reduce no-reflow area at 3 h of reperfusion significantly [(6.59±1.73)cm2 vs(4.68±1.53)cm2,P<0.05].Conclusion High dose of Tongxinluo could effectively reduce serum contents of adhesion and pro-inflammatory cytokines,regulate anti-inflammatory factor levels,and attenuate no-reflow area in the early reperfusion of AMI.It thus provided experimental basis for its clinical application. |
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