| 王卓,彭颖,李晓波.四君子汤对两种脾虚模型大鼠肠道菌群紊乱的影响[J].,2009,(9):825-829 |
| 四君子汤对两种脾虚模型大鼠肠道菌群紊乱的影响 |
| Effect of Sijunzi Decoction on the Intestinal Flora Disturbance in Two Rat Models of Pi-deficiency Syndrome |
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| DOI： |
| 中文关键词: 脾虚证 肠道菌群 四君子汤 |
| 英文关键词:Pi-deficiency syndrome intestinal microflora Sijunzi Decoction |
| 基金项目:国家自然科学基金重大研究计划资助项目(No.90209059,No.90409018) |
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| 中文摘要: |
| 目的观察两种脾虚模型大鼠肠道菌群的变化,研究四君子汤对肠道菌群紊乱的影响。方法实验动物分为番泻叶模型组和大黄模型组。收集造模前(健康)、造模后(脾虚)和治疗后的粪便样品,提取样品的肠道菌群总DNA并进行肠细菌基因间共有重复序列聚合酶链反应(ERIC-PCR),得到反映肠道微生物群结构的ERIC-PCR指纹图谱。采用Shannon-Weaver指数(多样性指数,H′)分析样品肠道菌群的多样性特征,Sorenson配对相似性系数(Cs)比较两个样品ERIC-PCR图谱间的相似性,用SPSS 11.5软件对所得数据进行t检验。结果两组造模前后肠道菌群明显改变,治疗后恢复到造模前水平。造模前后及治疗后各阶段粪便样品的肠道菌群H′番泻叶组分别为:2.20±0.12、2.00±0.10、2.27±0.10,大黄组分别为:2.09±0.02、1.88±0.10、1.94±0.10,差异均有统计学意义(P<0.05);Cs累积曲线分析表明,番泻叶组和大黄组<60%的Cs值造模前分别为0和19%,造模后为75%和52%,治疗后为4%和24%。结论两种脾虚型大鼠肠道菌群H′均显著性降低(P<0.05),治疗后H′均显著性增加(P<0.05),ERIC-PCR指纹图谱可作为脾虚证肠道菌群评价的分子指标。 |
| 英文摘要: |
| Objective To investigate the changes of intestinal microflora in two rat models of Pi-deficiency syndrome(PDS) to study the effect of Sijunzi Decoction(SJZD) on intestinal microflora alteration(IMA).Methods Wistar rats allocated in Group A and B were established into two kinds of PDS models by Folium Sennae and Radix et Rhizoma Rhei respectively.Fecal samples were collected from the model rats at three phases,i.e.before modeling,after modeling and after treated with SJZD.Total DNA of microbial communities in them was extracted and amplified with ERIC-PCR to generate fingerprints for reflect the intestinal microflora configuration.Shannon-Weaver index(H′) was used to analyze the diversity of rats′ intestinal microflora,Soreason pairwise similarity coefficient(Cs) was used to compare the similarity of different ERIC-PCR fingerprints,and Student′s t test was used for analyzing the statistical significance of their differences.Results The intestinal microflora in both groups obviously changed after modeling and recovered to the primary level after treated with SJZD.The H′ of the ERIC-PCR fingerprints at the three phases was 2.20±0.12,2.00±0.10 and 2.27±0.10 in Group A,and 2.09±0.02,1.88±0.10 and 1.94±0.10 in Group B respectively,both showing significant differences among different phases(P<0.05).Accumulative curves of Cs showed that the proportion of Cs accounting for less than 60% was 0 in Group A and 19% in Group B before modeling,75% and 52% for them respectively after modeling,and 4% and 24% after rehabilitation with SJZD.Conclusion The H′ of intestinal microflora in the two kinds of PDS models were significantly lowered,and could be recovered to the normal level by SJZD treatment(P<0.05).ERIC-PCR fingerprints can be used as a bio-criterion for evaluating intestinal microflora in patients with PDS. |
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