| 向阳,黄世林,陈楠楠,陈爱萍.补骨脂素加长波紫外线诱导NB4细胞凋亡及对Fas/FasL基因表达影响的研究[J].,2010,30(1):45-47 |
| 补骨脂素加长波紫外线诱导NB4细胞凋亡及对Fas/FasL基因表达影响的研究 |
| Effect of Combined Application of Psoralen and Ultraviolet A for Inducing NB4 Cell Apoptosis and Its Impact on Fas/FasL Gene Expressions |
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| DOI： |
| 中文关键词: 补骨脂素 长波紫外线 NB4细胞株 凋亡 Fas/FasL |
| 英文关键词:psoralen ultraviolet A NB4 cell line apoptosis Fas/FasL |
| 基金项目:国家自然科学基金资助项目(No.30472257) |
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| 中文摘要: |
| 目的探讨自中药补骨脂中提取的补骨脂素(psoralen,PSO)加长波紫外线(ultraviolet A,UVA)诱导人白血病细胞NB4凋亡及对Fas/FasL基因表达的影响。方法以NB4细胞为研究对象,采用流式细胞术、透射电镜、定量PCR等方法观察不同浓度的PSO,在不同时间的波长为360 nm的UVA照射下,细胞凋亡率、细胞超微结构以及细胞Fas/FasL基因、蛋白表达水平的变化。结果(1)补骨脂素加长波紫外线(PUVA)可诱导NB4细胞凋亡,当PSO浓度为10、20、40、80μg/mL时,UVA照射5 min的细胞凋亡率分别为(26.57±0.42)%、(30.67±0.11)%、(34.90±0.30)%、(24.63±0.38)%,凋亡率呈剂量-时间依赖性,两因素间有交互作用。(2)电镜下观察经PUVA处理后的NB4细胞超微结构,可见明显的凋亡形态学改变特征。(3)PUVA可上调NB4细胞Fas基因、蛋白的表达水平,并呈剂量-时间依赖性,在基因水平两因素间有交互作用,在蛋白水平两因素无交互作用。(4)PUVA可下调NB4细胞FasL基因、蛋白的表达水平,并呈剂量-时间依赖性,且两因素间有交互作用。结论PUVA可诱导白血病细胞NB4发生凋亡,Fas/FasL系统是PUVA诱导NB4细胞发生凋亡的作用途径之一。 |
| 英文摘要: |
| Objective To explore the effect of combined application of psoralen(PSO,an extract from psoralea) and ultraviolet A(UVA) for inducing the apoptosis of human leukemic cell line NB4 and its impact on the Fas/FasL gene expressions.Methods According to factorial design,changes of apoptosis rate and ultrastructure of NB4 cells,as well as the gene and protein expressions of Fas/FasL were observed after cells were treated with PSO in different concentrations and irradiated by UVA of 360 nm wavelength for different times,using flow cytometry,transmission electron microscopy and quantitative polymerase chain reaction(PCR),and the outcomes were treated with variable analysis.Results(1) After treatment of PSO in concentration of 10,20, 40,80μg/mL combined with a 5-min exposure of UVA,the NB4 cells apoptosis rate induced were 26.57%±0.42%,30.67%±0.11%,34.90%±0.30%and 24.63%±0.38%respectively.The effects were dose-and time-dependent,and an interaction was shown between the two actors.(2) After being treated by PSO plus UVA,obvious ultrastructure changes with apoptosis characteristics were shown in NB4 cell under electron microscope. (3) PSO plus UVA showed up-regulatory effect on gene and protein expressions of Fas,and down-regulatory effect on gene and protein expressions of FasL in a dose-and time-dependent manner,with the interaction between the two actors in altering Fas gene expression,also in altering FasL gene and protein expressions.Conclusion Combined application of PSO and UVA can induce the apoptosis of NB4 cells,and the Fas/FasL system is one of the pathways for apoptosis inducing. |
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