| 李雪梅,胡义扬,段小华,冯琴,彭景华,许丽莉,赵刚,刘平.基于均匀设计的抗肝纤维化中药有效组分配伍研究[J].,2010,30(1):58-63 |
| 基于均匀设计的抗肝纤维化中药有效组分配伍研究 |
| Uniform Designed Research on the Active Ingredients Assembling of Chinese Medicine Prescription for Anti-liver Fibrosis |
| |
| DOI： |
| 中文关键词: 扶正化瘀胶囊 中药有效组分/成分 配伍 肝纤维化 均匀设计法 |
| 英文关键词:Fuzheng Huayu Capsule active ingredients or constituents assembling liver fibrosis uniform design |
| 基金项目:教育部博士点基金(No.20070268002);国家科技部973计划(No.2006CB504801);上海市教委重点创新项目(No.08ZZ64);上海高校创新团队建设项目(第1期) |
|
| Hits: 1687 |
| Download times: 750 |
| 中文摘要: |
| 目的探索基于中药复方配伍理论的抗肝纤维化有效组分复方的研究方法和意义。方法以抗肝纤维化中药"扶正化瘀胶囊"中4种已知的有效组分或成分（虫草多糖、丹酚酸B盐、苦杏仁甙、绞股蓝总皂甙）为研究对象,分别运用二甲基亚硝胺（dimethylnitrosamine,DMN）和四氯化碳（CCl4）诱导的2种大鼠肝纤维化模型,采用均匀设计法"4因子8水平表"分组设计,治疗性给药;以大鼠肝组织胶原含量羟脯氨酸（Hyp）测定为筛选指标,并经均匀设计回归分析获得"最佳配方"。以上述2个不同模型研究获得的各自最佳配方为观察对象,以扶正化瘀胶囊以及四种有效组分合方（最佳配方加被筛除的有效组分）为对照,运用上述2种动物模型,通过观察肝组织Hyp含量和血清ALT活性检测和肝组织纤维化的变化,对所得配方的治疗效果再次进行比较和验证实验。结果（1）DMN模型试验筛选的A配方（虫草多糖60 mg、苦杏仁甙80 mg、绞股蓝总皂甙50 mg）和CCl4模型试验筛选的B配方（虫草多糖20 mg、苦杏仁甙160 mg、绞股蓝总皂甙50 mg）皆提示由虫草多糖、苦杏仁甙、绞股蓝总皂甙3种有效组分配伍为最佳,但两者量比不同;（2）验证实验显示A配方和B配方均疗效显著,与扶正化瘀胶囊相当（P>0.05）,而A配方和B配方添加被筛除的丹酚酸B盐后,显示疗效下降。结论（1）均匀设计在中药有效组分或成分配伍研究中有重要应用价值;（2）基于中药传统复方的有效组分的合理配伍可以达到"重现原复方效应"的目的;（3）有效组分或成分配伍,有内在的量变和质变规律,有"增效"也有"拮抗"现象,不能简单堆砌。 |
| 英文摘要: |
| Objective To explore the method and significance for studying active anti-liver fibrosis ingredients consisted Chinese medicine compound prescription based on Chinese medicine theory.Methods Optimized prescription was screened out,adopting uniform block design with 4-factor 8-level table and regression analysis, through applying the four known effective ingredients（cordyceps sinensis polysaccharide,salvianolic acid B,amygdaloside and gypenosides） of Fuzheng Huayu Capsule（FZHYC,a new Chinese medine anti-liver fibrosis drug） to two rat liver fibrosis models established separately by dimethylnitrosamine（DMN） and CCl4, and taking the liver content of hydroxyproline（Hyp） as the screen index.Then a further study for comparing and verifying the efficacy of the obtained optimized prescription was conducted on the two former models respectively by observing the changes of Hyp content in liver,serum ALT activity and fibrosis pathology after medication,controlled by the original FZHYC and the recipe assembled by all the four ingredients.Results Two optimized prescriptions （OPA and OPB） were screened out separately in the studies conducted on the two models.Both of them were consisted of cordyceps sinensis polysaccharide,Amygdaloside and Gypenosides,but different in constituent ratio,i.e.,the ratio in OPA was 60:80:50,and that in OPB,20:160:50.Verifying study showed both OPA and OPA were significantly effective,with the efficacy equivalent to that of FZHYC（P>0.05）.However, when they were used in combining with salvianolic acid B（the cutout ingredient in the screening）,the efficacy lowered surely.Conclusions Uniform design is a valuable method in the compatibility research of Chinese Medicine drugs’ composition.To assemble a new compound recipe reasonably based on the prescription of traditional compound recipe could make its effect equivalent to that of the original prescription.Ingredients or constituents in a prescription,either presented synergistic or antagonistic effects,are not randomly stacked together,and they should be orderly assembled in intrinsic rules of qualitative and quantitative changing. |
| View Full Text View/Add Comment Download reader |
|
|
|
