| 刘兆华,程芳,周庚寅,刘兆平.C57BL/6J小鼠胭窝淋巴结试验评价注射用双黄连的致敏性研究[J].,2010,30(1):64-67 |
| C57BL/6J小鼠胭窝淋巴结试验评价注射用双黄连的致敏性研究 |
| Study on the Sensitizing Potential of Shuanghuanglian Injection Using Poplite Lymph Node Assay in C57BL/6J Mice |
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| DOI： |
| 中文关键词: 腘窝淋巴结试验 注射用双黄连 致敏性评价 |
| 英文关键词:popliteal lymph node assay Shuanghuanglian Injection sensitization assessment |
| 基金项目:国家科技支撑计划项目(No.2006BA114B05) |
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| 中文摘要: |
| 目的比较注射用双黄连和致敏物引起的腘窝淋巴结（popliteal lymph node,PLN）反应,探讨注射用双黄连的致敏潜能。方法雌性C57BL/6J小鼠随机分为空白对照（Veh）组、苯巴比妥（PB）组（1 mg/只）、HgCl2组（50μg/只）和D-青霉胺（D-pen）组（2 mg/只）、注射用双黄连低剂量组（L组,1 mg/只）和高剂量组（H组,5 mg/只）,每组10只。小鼠一侧足趾部皮下给予受试物,第8天处死动物,摘取两侧PLN,称重,计算质量指数（mass index,MI）;每组将4只小鼠的PLN以4%中性多聚甲醛固定用于病理学检查,其余6只制备单细胞悬液,计算细胞指数（cells index,CI）,比较PLN的变化。结果H组MI、CI较Veh组升高（P<0.05）,MI≥2和CI≥5。病理检查,PLN体积变大,生发中心明显,副皮质区内高内皮静脉（HEV）横断面增多。结论注射用双黄连可诱导C57BL/6J小鼠产生明显的PLN反应,提示它具有一定的致敏潜能。 |
| 英文摘要: |
| Objective To investigate the sensitizing potential of Shuanghuanglian Injection（SHL） by comparing the popliteal lymph node（PLN） response in mice induced by SHL and chemicals.Methods Sixty female C57BL/6J mice were equally and randomly divided into six groups,i.e.the blank control group（A） and five treated groups treated respectively with phenobarbital 1 mg/mouse（B）,mercuric chloride（Hgcl2） 50μg/ mouse（C）,D-penicillamine 2 mg/mouse（D）,and SHL in low（1 mg/mouse） and high（5 mg/mouse） dosages （E and F） via subcutaneous injection into left pad of hind foot.Animals were sacrificed on the 8th day after injection,their bilateral PLNs were isolated and weighed respectively to calculate the PLN mass index（MI）. Then the PLNs get from four mice in each group were fixed with 4%paraformaldehyde solution for histopathologic examination;the other six PLNs were prepared into single-cell suspensions to calculate cell index（Cl） for comparing the changes of PLN in various groups.Results MI and CI in Group F reached to≥2 and≥5（average） respectively,which was higher than those in Group A（P<0.05）.Pathological examination showed that the left PLN in Group F enlarged,with remarkable germinal center and increased high endothelial venules proliferation. Conclusion SHL could induce significant PLN response in C57BL/6J mice,suggesting it has certain sensitizing potential. |
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