| 赵永华,刘煜德,刘乃维,李焕仪,项平.颐年降压饮含药血清对自发性高血压大鼠内皮细胞增殖及PPAR-γ mRNA表达的影响[J].,2010,30(1):68-71 |
| 颐年降压饮含药血清对自发性高血压大鼠内皮细胞增殖及PPAR-γ mRNA表达的影响 |
| Effects of Yinian Jiangya Decoction Contained Serum on Endothelial Cell Proliferation and PPARγ mRNA Expression in Spontaneously Hypertensive Rat |
| |
| DOI： |
| 中文关键词: 颐年降压饮 自发性高血压 内皮细胞增殖 过氧化物酶体增殖物激活受体γ |
| 英文关键词:Yinian Jiangya Decoction spontaneous hypertension endothelial cell proliferation peroxisome proliferator-activated receptor-γ |
| 基金项目:澳门特别行政区科学技术发展基金资助课题(No.05/2005/A) |
|
| Hits: 1652 |
| Download times: 754 |
| 中文摘要: |
| 目的观察颐年降压饮含药血清对体外原代培养的自发性高血压大鼠(spontaneously hypertensiverats,SHR)内皮细胞增殖及PPAR-γmRNA表达的影响。方法取SHR主动脉内皮细胞进行原代培养,取3代后内皮细胞用于实验,SD大鼠40只随机分为正常血清对照组和颐年降压饮高、中、低剂量组,给与高脂饮食喂养,分别灌服生理盐水和高、中、低剂量颐年降压饮(分别含生药5.2 g/mL、2.6 g/mL、1.3 g/mL),给药20天麻醉后开始收集血清,经灭活后作用各组内皮细胞。MTT法检测不同浓度血清作用各组2、4、8、16、24、48 h细胞活性;RT-PCR法检测作用4、8、16、24 h内皮细胞PPAR-γmRNA表达情况。结果MTT检测OD值发现,4、8 h颐年降压饮高、中、低剂量组OD值均高于正常血清对照组,差异有统计学意义(P<0.05),而颐年降压饮3剂量组间差异无统计学意义(P>0.05);16 h颐年降压饮中剂量组OD值上升低于其他组,差异有统计学意义(P<0.05);24 h各组OD值均下降,但颐年降压饮高、中剂量组下降比低剂量、正常血清对照组明显,差异有统计学意义(P<0.05);48 h各组OD值继续下降,颐年降压饮高剂量组比正常血清对照组下降更明显(P<0.05)。RT-PCR检测不同血清作用内皮细胞4 h时,各组PPAR-γmRNA表达差异无统计学意义(P>0.05);8 h时颐年降压饮高剂量组PPARγmRNA表达明显低于其他各组(P<0.05);16 h颐年降压饮各剂量组PPAR-γmR NA表达比正常血清对照组高(P<0.05),其中颐年降压饮高剂量组PPARγmR NA表达最高,与其他组比较,差异有统计学意义(P<0.01);24 h各组PPAR-γmR NA表达有所下降,但颐年降压饮低剂量组PPAR-γmRNA表达仍高于正常血清对照组(P<0.05)。结论颐年降压饮对内皮细胞增殖呈双向调节作用,或许与调节PPAR-γmR NA表达有关。颐年降压饮所具有的上调及维持PPAR-γmRNA表达作用,可能是该方药具有保护血管内皮功能,降低血压的机制之一。 |
| 英文摘要: |
| Objective To observe the effects of Yinian Jiangya(YNJY) Decoction contained serum on cell proliferation of primary cultured endothelial cells of spontaneously hypertensive rat(SHR) and the peroxisome proliferator-activated receptor-γ(PPAR-γ) mRNA expression in the cells.Methods SD rats were fed with high-lipid diet and different doses(high,medium and low,containing crude drug of 5.2 g/mL,2.6 g/mL, 1.3 g/mL respectively) of YNJY respectively and the serum contained different doses of YNJY(S-YNJY) was prepared by collecting the rats’ serum after 20 days of feeding.The serum obtained from SD rats fed with normal diet,i.e.drug free serum(S-free) was taken for control.Primary cultured endothelial cells of SHR were treated respectively with S-YNJY in different doses(treated groups) and S-free(control group),the cell activity and the mRNA expression of PPARγin cells of all groups were detected by MTT and RT-PCR respectively at different time points:2,4,8,16 and 24 h after treatment.Results MTT test showed that at time points of 4 and 8 h,the OD value raised in the treated groups with insignificant difference among them(P>0.05),but was higher than that in the control group(P<0.05);at 16 h,it increased but showed a smaller increment in the medium-dose treated group(P<0.05);at 24 h,it decreased in all groups,but the decrement in the high-and medium-dose treated groups was more remarkable(P<0.05);at 48 h,it decreased continuously,with the decrement more significant in the high-dose group than in the control group(P<0.05).RT-PCR detection showed that at 4 h, the expression of PPARγmRNA was not changed significantly in all groups(P>0.05);at 8 h,it was remarkably lower in high-dose group than in others(P<0.05);at 16 h,it was higher in the three treated groups than in the control group(P<0.05),with a highest level shown in the high-dose treated group(P<0.01);at 24 h,the expression decreased in all groups,but the level in the low-dose treated group was still higher than that in the control group(P<0.05).Conclusions YNJY Decoction shows a two-way regulation on endothelial cells proliferation, and which is negatively dose-dependent in the late stage.The regulation is perhaps relevant to the regulating of the PPARγmRNA expressions.The PPARγmRNA expression up-regulating and maintaining effects of YNJY Decoction may be one of the mechanisms for its vascular endothelial cell protection and blood pressure suppressing. |
| View Full Text View/Add Comment Download reader |
|
|
|
