| 张才擎,梁铁军,张心月,邵雨萌,何荣,张敏,龙飞,李道卫.温肺化饮方对慢性阻塞性肺疾病大鼠肺保护作用的实验研究[J].,2010,30(1):72-75 |
| 温肺化饮方对慢性阻塞性肺疾病大鼠肺保护作用的实验研究 |
| Protective Effect of Wenfei Huayin Recipe on Lung of Rats with Chronic Obstructive Pulmonary Diseases |
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| DOI： |
| 中文关键词: 温肺化饮方 寒痰蕴肺型 慢性阻塞性肺疾病 |
| 英文关键词:Wenfei Huayin Recipe cold-phlegm retention in Fei syndrome type chronic obstructive pulmonary disease |
| 基金项目:山东省科技厅科技攻关计划项目(No.2002-17) |
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| 中文摘要: |
| 目的探讨温肺化饮方治疗慢性阻塞性肺疾病（COPD）的机制。方法采用改良烟熏加气管滴加脂多糖方法复制COPD模型,二次造模复制成寒痰蕴肺型COPD。然后随机分为治疗组、模型组,治疗组灌服温肺化饮方,连续14天;模型组大鼠每天予2 mL生理盐水灌胃;另设空白对照组不做任何干预。观察大鼠一般情况,体重增长指数,光学显微镜下观察肺组织形态学改变,电子显微镜观察超微结构,以及大鼠动脉血气分析,放射免疫法检测肺组织匀浆白细胞介素（IL）-4、IL-8、肿瘤坏死因子-α（TNF-α）、γ干扰素（IFN-γ）含量。结果治疗组大鼠扎堆恶寒现象、哮鸣音等症状及各级气道上皮细胞变性坏死、慢性炎性细胞浸润程度较模型组明显减轻;纤毛倒伏、脱落及肺大泡数量较模型组显著减少;治疗组大鼠体重增长指数较模型组显著上升（P<0.01）;治疗组降低动脉血二氧化碳方压（PaCO2）、IL-4、IL-8、TNF-α水平,升高动脉血氧分压（PaO2）、IFN-γ水平及IFN-γ/IL-4比值明显优于模型组（P<0.01,P<0.05）。结论温肺化饮方能纠正Th1/Th2比值的失衡,抑制炎性反应,对于COPD大鼠气道功能、结构改善起着非常重要的作用。 |
| 英文摘要: |
| Objective To explore the mechanism of Wenfei Huayin Recipe（WHR） in treating chronic obstructive pulmonary diseases（COPD）.Methods The COPD model was induced by modified fumigating method and intra-tracheal instillation of lipopolysaccharide.Then reformed COPD model of cold-phlegm retention in Fei syndrome type.All the model rats were randomly divided into two groups,the model group and the treated group,treated respectively with WHR and saline for 14 successive days.Besides,a blank group without any intervention was set up for control.The general condition,weight growth rate,pathological changes of lung tissue under light microscope,ultrastructure under electron microscope,arterial blood gas analysis and levels of interleukin 4（IL-4）,interleukin 8（IL-8）,tumor necrosis factorα（TNF-α） and interferonγ（IFN-γ） in lung homogenate by radio-immunity assay were observed.Results In the treated group,as compared with the control group,the symptoms of aversion to cold,swarming,wheezing,the degree of epithelial cell degeneration and necrosis,the inflammatory cell infiltration and the volume of cilia lodging,sloughing,and bullae of lung were lessened and weight growth rate was higher（P<0.01）.Moreover,the treated group was superior to the control group in decreasing levels of PaCO2,IL-4,IL-8,TNF-αand increasing PaO2,IFN--γand IFN-γ/IL-4 ratio（P<0.01 or P<0.05）.Conclusion WHR can correct the Th1/Th2 imbalance and inhibit the inflammatory reaction, displaying an important role in improving the airway function and structure in COPD rats. |
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