| 荀运浩,施军平,过建春,石伟珍,刘长灵.中医体质和HBV感染结局的关联及其与人类白细胞抗原-DQA1基因多态性的关系[J].,2010,30(2):141-145 |
| 中医体质和HBV感染结局的关联及其与人类白细胞抗原-DQA1基因多态性的关系 |
| Association of Chinese Medicine Constitution and Human Leukocyte Antigen-DQA1 Gene Polymorphism with Outcomes of Hepatitis B Virus Infection |
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| DOI： |
| 中文关键词: 乙型肝炎 病毒性 中医体质 人类白细胞抗原 基因多态性 |
| 英文关键词:hepatitis B viral Chinese medicine constitution type human leukocyte antigen polymorphism of gene |
| 基金项目:浙江省中医药科技计划项目(No.2007CB199) |
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| 中文摘要: |
| 目的观察中医体质类型与浙江地区汉族人群乙型肝炎病毒(HBV)感染不同临床状态及人类白细胞抗原(HLA)DQA1基因多态性的关系,探讨中医体质因素在慢性乙型肝炎发病中的作用。方法临床收集慢性乙型肝炎(CHB,120例)、慢性HBV携带者(ASC,60例)、自限性HBV感染者(RHBS,60例)3组患者,前两组诊断均经肝活检证实。以王琦体质分类判定中医体质类型;聚合酶链反应序列特异性引物(PCR-SSP)法检测HLA-DQA1基因型,比较组间体质类型分布的差异及组间基因频率的差异。结果(1)CHB组阴虚质、痰湿质的分布频率(20.0%、12.5%)显著高于RHBS组(6.7%、1.7%),平和质的分布频率(11.7%)显著低于RHBS组(31.7%),差异均有统计学意义(OR=3.500,95%CI:1.16-10.60;OR=8.400,95%CI:1.09-65.42;OR=0.161,95%CI:0.076-0.34;均P<0.05);(2)CHB组湿热质的分布频率(24.2%)显著高于ASC组(6.7%,P<0.05,OR=4.462,95%CI:1.49-13.36),平和质的分布频率(11.7%)显著低于ASC组(45.0%,P<0.01,OR=0.285,95%CI:0.13-0.62);(3)HLA-DQA1*0201在CHB组的分布频率(38.3%)显著高于RHBS组(5.8%,P<0.01,OR=10.04,95%CI:4.48-22.48);HLA-DQA1*0102的分布频率(9.6%)显著低于RHBS组(36.7%,P<0.01,OR=0.183,95%CI:0.10-0.32);(4)HLA-DQA1*0201在CHB组的分布频率(38.3%)显著高于ASC组(7.5%,P<0.01,OR=7.667,95%CI:3.7-15.87);HLA-DQA1*0102的分布频率(20%)显著低于ASC组(9.6%,P<0.01,OR=0.424,95%CI:0.23-0.79)。结论中医体质因素和HLA-DQA1基因多态性均可影响HBV感染的临床结局,但其间关系需进一步研究明确。 |
| 英文摘要: |
| Objective To observe the Chinese medicine constitution types and human leukocyte antigen (HLA)-DQA1 gene polymorphism in patients with hepatitis B (HB) virus infection in Chinese Han population of Zhejiang Province, for exploring the roles of constitution factor in pathogenesis of HB. Methods A total of 240 subjects, including 120 biopsy-proven chronic HB (CHB), 60 HB asymptomatic carrier (ASC) and 60 resolved from HBV infection spontaneously (RHBS) were studied. Their Chinese medicine constitution type was judged by Wangqi′s classification, and their genotype of HLA-DQA1 was detected by polymerase chain reaction sequence specific primer for comparing the difference between groups in distribution frequency (DF) of constitution types and genes.Results (1) As compared with the RHBS group, DF of yin-deficiency constitution and phlegm-dampness constitution in the CHB group was significant higher (20.0% vs 6.7% and 12.5% vs 1.7%), and that of placid constitution was significant lower (11.7% vs 31.7%), showing statistical significance between groups (OR=3.5, 95%CI: 1.16-10.60; OR=8.4, 95%CI: 1.09- 65.42; OR=0.161, 95%CI: 0.076-0.34; all P<0.05). (2) As compared with the ASC group, DF of damp-heat constitution was significant higher (24.2% vs 6.7%, P<0.05, OR=4.462, 95%CI: 1.49-13.36), and that of placid constitution was significant lower (11.7% vs 45.0%, P<0.01, OR=0.285, 95%CI: 0.13-0.62) in the CHB group. (3) As compared with RHBS group, DF of HLA-DQA1 0201 allele in CHB group was significant higher (38.3% vs 5.8%, P<0.01, OR=10.04, 95%CI: 4.48- 22.48); and that of HLA-DQA10102 allele was significant lower (9.6% vs 36.7%, P<0.01, OR=0.183, 95%CI: 0.10-0.32). (4) As compared with ASC group, DF of HLA-DQA10201 allele in CHB group was significant higher (38.3% vs 7.5%, P<0.01, OR=7.667, 95%CI: 3.7-15.87), and that of HLA-DQA10102 allele was significant lower (20.0% vs 9.6%, P<0.01, OR=0.424, 95%CI: 0.23-0.79). Conclusion Both Chinese medicine constitution and HLA-DQA1 gene polymorphism show connection with the outcomes of HB virus infection in Chinese Han population, but the real association between them is required for further study. |
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