| 王颖,马东丽,接英,潘志强,徐健,武宇影.中药菊花决明散防治大鼠高危角膜移植免疫排斥反应的研究[J].,2010,30(2):178-181 |
| 中药菊花决明散防治大鼠高危角膜移植免疫排斥反应的研究 |
| Effect of Juhua Jueming Powder in Preventing High-risk Corneal Transplantation Immune Rejection in Rats |
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| DOI： |
| 中文关键词: 高危角膜移植 免疫排斥 菊花决明散方 |
| 英文关键词:high-risk corneal transplantation immune rejection Juhua Jueming powder |
| 基金项目:国家自然科学基金资助项目(No.30672712) |
| Author Name | Affiliation | | WANG Ying | 首都医科大学附属北京同仁医院,北京同仁眼科中心,北京市眼科学与视觉科学重点实验室 | | MA Dong-li | 首都医科大学附属北京同仁医院,北京同仁眼科中心,北京市眼科学与视觉科学重点实验室 | | JIE Ying | 首都医科大学附属北京同仁医院,北京同仁眼科中心,北京市眼科学与视觉科学重点实验室 | | 潘志强 | 首都医科大学附属北京同仁医院,北京同仁眼科中心,北京市眼科学与视觉科学重点实验室 | | 徐健 | 首都医科大学附属北京同仁医院,北京同仁眼科中心,北京市眼科学与视觉科学重点实验室 | | 武宇影 | 首都医科大学附属北京同仁医院,北京同仁眼科中心,北京市眼科学与视觉科学重点实验室 |
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| 中文摘要: |
| 目的探讨中药菊花决明散对大鼠高危角膜移植排斥反应的抑制作用。方法角膜缝线法诱导受体角膜新生血管,行穿透性角膜移植,建立大鼠高危角膜移植排斥反应模型。术后治疗组采用每天菊花决明散方0.1g/2mL灌胃,对照组采用生理盐水2mL灌胃,观察植片存活状况;应用组织病理学、免疫荧光组织化学,流式细胞仪等方法对受体局部和全身免疫状态进行检测。结果治疗组大鼠角膜植片存活时间为(14.50±3.55)天,明显长于生理盐水组的(8.25±0.71)天(P<0.01);治疗组术后虹膜铺片Fas、FasL的表达低于对照组;术后14天外周血中CD4+CD2+5FOXP3+阳性细胞治疗组[(14.81±2.58)%]高于对照组[(5.11±3.92)%],差异有统计学意义(P<0.01);治疗组房水中IL-2浓度低于对照组而IL-10浓度高于对照组。结论菊花决明散方对高危角膜移植排斥反应模型大鼠术后免疫排斥反应具有一定抑制作用。 |
| 英文摘要: |
| Objective To study the inhibitory effect of Juhua Jueming powder (JJP) on high-risk corneal transplantation immune rejection in rats. Methods The high-risk corneal transplantation immune rejection rat model was established by inducing coneal neoangiogenesis by suture method and the penetrating transplantation. Model rats were divided into two groups, the treated group and the control group, they were administered with JJP 0.1 g (dissolved in 2 mL water) and normal saline respectively via gastric infusion every day after transplantation. The survival status of the allograft, histopathology, local and systemic immune status in the recipients were observed using immunofluorescence histochemistry and flow cytometry. Results The survival time of the allograft in the treated group (14.50±3.55 days) was significantly longer than that in the control group (8.25±0.71 days, P<0.01). Levels of Fas, FasL expressions in iris were stronger, and the percentage of CD+4CD+25FOXP3 positive cells in peripheral blood was less (5.11±3.92% vs 14.81±2.58%) in the control group than those in the treated group respectively. The concentration of IL-2 was lower while that of IL-10 was higher in aqueous humor of the treated group than those of the control group, respectively. Conclusion JJP has certain effect for preventing high-risk corneal transplantation immune rejection in rat model. |
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