| 张文富,凌江红,王爱坚,黄李平,杨靖,李山青.广西巴马长寿老人肾虚证候与ACE基因多态性的相关性研究[J].,2010,30(3):259-263 |
| 广西巴马长寿老人肾虚证候与ACE基因多态性的相关性研究 |
| Relationship between Shen-deficiency Syndrome and Polymorphism of Angiotensin Converting Enzyme Gene in Longevous Elders in Bama Area of Guangxi Municipality |
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| DOI： |
| 中文关键词: 长寿 肾虚证候 血管紧张素转换酶基因 |
| 英文关键词:longevity Shen-deficiency syndrome angiotensin converting enzyme gene |
| 基金项目:广西医科大学博士启动基金项目(No.308047);广西医科大学研究生教育创新课题项目(No.2008105981006M222) |
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| 中文摘要: |
| 目的探讨广西巴马长寿地区老年人肾虚证候与血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性的关系。方法采用问卷调查并采血进行聚合酶链反应(PCR)、单链构象多态性分析(SSCP)与直接测序技术检验确认,研究百岁组(≥100岁)27名、90岁组(90~99岁)56名、老年组(60~89岁)114名老人的肾虚证候及其与ACE基因I/D多态性的关系,以79例60~70岁自然死亡人群为对照。结果D/D型老年人肾虚症状最少,I/D型老年人次之,I/I型老年人肾虚症状最多。在ACE基因I/D多态基因型中,百岁组I/I、I/D、D/D型频率分别为37.04%(10/27)、33.33%(9/27)和29.63%(8/27),I和D等位基因频率分别为53.70%(29/54)和46.30%(25/54)。百岁组D/D基因型频率和D型等位基因频率高于老年组及对照组(P<0.05,P<0.01),但与90岁组比较,无论是I/I、I/D或D/D基因型,所占百分率均比较接近,差异无统计学意义(P>0.05)。D/D基因型经SSCP证实全部相同,无差别。直接测序结果再次验证ACE(I/D)多态性判断准确。结论老年肾虚证候与ACE基因I/D多态性及长寿密切相关,同时也应考虑到长寿受多种因素影响。 |
| 英文摘要: |
| ObjectiveTo explore the relationship between Shen-deficiency syndrome(SDS) and the insert/deficit(I/D) polymorphism types(PMTs) of angiotensin converting enzyme(ACE) gene in longevous elders in Bama area of Guangxi municipality.MethodsThe investigation on 27 centenarians(Group A),56 elders of 90-99 years old(Group B) and 114 of 60-89 years old(Group C) was carried out by questionnaire,and the polymorphism was detected using polymerize chain reaction detection,single strand conformation polymorphism(SSCP) analysis,and direct sequencing technique.And the study was controlled by 79 naturally died elders within 60-70 years old.ResultsThe presenting frequencies of SDS in various PMT were D/D,I/D and I/I in rising order.The frequencies of PMT,I/I,I/D and D/D,were 37.04%(10/27),33.33%(9/27),and 29.63%(8/27) respectively;the frequency rates of I and D alleles were 53.70%(29/54) and 46.30%(25/54) in Group A.Comparison of frequency of D/D and D allele between groups showed that Group A was significantly different to Group C(P<0.05) and also to the control(P<0.01),while all the frequencies in Group A and B were rather identical,showing insignificant difference(P>0.05).SSCP analysis showed no significant difference in D/D among groups.Outcomes of direct sequencing test further proved the correctness of I/D ACE polymorphism detection.ConclusionSDS is closely correlated with ACE gene polymorphism and life span.Whereas,other multiple factors that influence longevity should also be taken into consideration. |
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