| 赵薇,温海霞,郑慧丽,孙世晓,倪江.左归饮对围绝经期大鼠卵巢促卵泡激素受体表达的影响及机制[J].,2010,30(3):286-290 |
| 左归饮对围绝经期大鼠卵巢促卵泡激素受体表达的影响及机制 |
| Effect of Zuoguiyin on the Expression of Ovarian Follicle-stimulating Hormone Receptor in Rats during Peri-menopausal Period |
| |
| DOI： |
| 中文关键词: 左归饮 促卵泡激素受体 围绝经期 卵巢 大鼠 |
| 英文关键词:Zuoguiyin follicle-stimulating hormone receptor peri-menopausal period ovary rat |
| 基金项目:哈尔滨市科技攻关资助项目(No.2005AA9CS116-17);黑龙江省中医药大学科研基金资助项目(No.200722) |
|
| Hits: 1532 |
| Download times: 829 |
| 中文摘要: |
| 目的探讨左归饮对围绝经期大鼠卵巢促卵泡激素受体(follicle-stimulating hormone receptor,FSHR)表达的影响及机制。方法采用围绝经期大鼠动物模型,分别给予低、中、高剂量左归饮灌胃处理8周,用RT-PCR和免疫组化法检测卵巢FSHR mRNA及蛋白的表达。未成年大鼠注射孕马血清后收集培养卵巢颗粒细胞,用RT-PCR方法检测给予不同处理因素后FSHR mRNA的表达水平。结果(1)围绝经期大鼠卵巢FSHR的表达明显低于青年组(P<0.01),给予不同剂量左归饮处理后,FSHR表达均显著升高(P<0.01)。(2)培养颗粒细胞给予不同处理因素,结果显示,与对照组相比,左归饮提取物可促进FSHR mRNA表达(P<0.01);与单纯加入左归饮提取物相比,同时加入左归饮提取物和福司考林(Forskolin)可进一步促进FSHR mRNA的表达(P<0.01),同时加入左归饮提取物和酪氨酸蛋白激酶抑制剂Genistein则能明显削弱左归饮的促进作用。结论左归饮可通过上调围绝经期大鼠卵巢FSHR的表达,提高卵巢对卵泡刺激素的反应性,改善围绝经期卵巢功能。左归饮对FSHR mRNA表达的促进作用可能是通过激活腺苷酸环化酶-蛋白激酶A(cAMP-PKA)及酪氨酸蛋白激酶途径实现的。 |
| 英文摘要: |
| ObjectiveTo investigate the effect and mechanism of Zuoguiyin(ZGY,a Chinese medical remedy for regulating qi and nourishing yin) on expression of ovarian follicle-stimulating hormone receptor(FSHR) in rats during peri-menopausal period(PMP).MethodsPMP rats were administered with low(13.78 g/kg),middle(20.67 g/kg) and high(31.00 g/kg) dose ZGY respectively by gastrogavage for eight weeks.FSHR mRNA and protein expressions were detected by RT-PCR and immunohistochemistry respectively.Besides,granulosa cells,collected from pregnant mare serum treated nonage rats,were incubated,and the level of FSHR mRNA expression in the cultured cells were detected after various disposals.Results(1) Ovarian levels of FSHR mRNA and protein expressions in PMP rats were significantly lower than those in young rats(P<0.01),but were up-regulated significantly by ZGY treatment(P<0.01).(2) As compared with the control,FSHR mRNA expression increased in cultured granulosa cells after treated by ZGY extract;the increasing effect of ZGY was enhanced by combined treatment with Forskolin and attenuated by Genistein(a tyrosine protein kinase inhibitor).ConclusionZGY could improve the ovarian functions during PMP by up-regulating the expression of FSHR and raise the ovarian responsibility to FSH,which may be possibly realized by activating cAMP-protein kinase and tyrosine protein kinase signal pathway. |
| View Full Text View/Add Comment Download reader |
|
|
|
