| 邓聪,魏连波,黄丽雯,李玉明,龚春水.肾衰养真胶囊对慢性肾衰竭营养不良大鼠Leptin-下丘脑NPY、POMC轴的影响[J].,2010,30(9):952-956 |
| 肾衰养真胶囊对慢性肾衰竭营养不良大鼠Leptin-下丘脑NPY、POMC轴的影响 |
| Effects of Shenshuai Yangzhen Capsule on Hypothalamic Leptin-Neuropeptide and Proopiomelanocortin Axes in Chronic Renal Failure Rats with Malnutrition |
| |
| DOI： |
| 中文关键词: 肾衰养真胶囊 慢性肾衰竭 营养不良 瘦素 神经肽Y 阿片-促黑素细胞皮质素原 |
| 英文关键词:Shenshuai Yangzhen Capsule chronic renal failure malnutrition leptin neuropeptide Y proopiomelanocortin |
| 基金项目:国家自然科学基金资助项目(No.30672768);广东省自然科学基金资助项目(No.06024428);广州市科技基金资助项目(No.2006Z3-E5051-1);南方医科大学开发基金资助项目(No.200601);珠江医院院长基金资助项目(No.200302) |
|
| Hits: 1435 |
| Download times: 595 |
| 中文摘要: |
| 目的观察肾衰养真胶囊对慢性肾衰竭(chronic renal failure,CRF)营养不良大鼠瘦素(leptin,LP)-下丘脑神经肽Y(neusropeptide Y,NPY)及阿片-促黑素细胞皮质素原(proopiomelanocortin,POMC)轴的影响。方法 42只SD雄性SPF级大鼠,随机选取正常组8只,其余大鼠采用5/6肾切除同时予4%酪蛋白饮食制作CRF营养不良大鼠模型,观察其营养不良发生时间,随机分为肾衰养真胶囊组(SSYZC组)11只、复方α-酮酸组(对照组)11只、模型组12只。治疗4周后,检测肌酐(serum creatinine,Scr)、尿素氮(blood urea nitrogen,BUN)、24小时尿蛋白(24 hour urine protein,24 h Upr)、白蛋白(albumin,ALB)、血红蛋白(haemoglobin,Hb)、胰岛素样生长因子-1(type-1 insulin like growth factor,IGF-1)、总胆固醇(total cholesterol,TC)、甘油三脂(triglyeride,TG),动态观察摄食量及体重变化,应用放射免疫法检测血浆瘦素(leptin,LP)、下丘脑神经肽Y(neusopeptide Y,NPY)和RT-PCR法检测下丘脑OB-Rb mRNA、NPYmRNA和POMCmRNA表达水平。结果 CRF大鼠在术后10周末出现营养不良,SSYZC组大鼠摄食量、体重显著增高(P<0.05),肾功能及营养状况明显改善(P<0.05),肾衰养真胶囊可降低血浆LP、NPY水平(P<0.05);可上调下丘脑NPY mRNA表达(P<0.01)及下调OB-Rb mRNA、POMC mRNA表达(P<0.01)。结论肾衰养真胶囊可能通过上调下丘脑NPY mRNA表达及下调OB-Rb mRNA、POMC mRNA表达,从而促进大鼠摄食,改善CRF营养不良。 |
| 英文摘要: |
| Objective To investigate the effect of Shenshuai Yangzhen Capsule(SYC) on hypothalamic leptin-neuropeptide Y(NPY) and proopiomelanocortin(POMC) axes in chronic renal failure(CRF) rats with malnutrition(MN).Methods Forty-two male SD rats of SPF grade were established into CRF-MN model by 5/6 nephrectomy and 4%casein diet,the happening time of MN in them was recorded.Rats successfully modeled were randomized into three groups,11 rats in Group A treated with SYC,11 in group B treated with compositeα-keto acid and 12 in Group C was untreated.Besides,a normal control group was set up with 8 healthy rats.After being treated for 4 weeks,the renal function related indices,including serum creatinine(Scr),blood urea nitrogen (BUN),24 hour urine protein(24 h Upro),albumin(ALB),haemoglobin(Hb) insulin like growth factor-1 (IGF-1),total cholesterol(TC) and triglyeride(TG) were measured,and body weight,food intake in rats were observed dynamically,blood leptin and NPY level in rats were determined by radioimmunoassay;mRNA expressions of OB-Rb,NPY and POMC in hypothalamus were detected with RT-PCR.Results CRF rats revealed MN at the end of 10th week after modeling.Compared with Group C,the condition of MN in Group A was significantly improved,showing increase of food intake and body weight(P<0.05),marked improvement of renal function (P<0.05),decrease of LP and NPY levels in plasma(P<0.05),as well as up-regulated NPY mRNA expression and down-regulated mRNA expressions of OB-Rb and POMC in hypothalamus(P<0.01 ). Conclusion SYC can improve the malnutrition condition in rats with CRF,which is possibly by way of depressing OB-Rb and POMC mRNA expression and upgrading NPY mRNA expression in hypothalamus. |
| View Full Text View/Add Comment Download reader |
|
|
|
