| 仇琪,李春,王勇,陈建新,郭淑贞,赵慧辉,王伟.心肌缺血模型大鼠血瘀证相关信息的评价[J].,2010,30(9):974-977 |
| 心肌缺血模型大鼠血瘀证相关信息的评价 |
| Evaluation of Blood-stasis Pattem Related Information in Myocardial Ischemic Rat Model |
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| DOI： |
| 中文关键词: 心肌缺血 血瘀证 大鼠 |
| 英文关键词:myocardial ischemia blood stasis pattern rat |
| 基金项目:国家重大新药创制计划新药研究开发关键技术研究(No.2009ZX09502-018) |
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| 中文摘要: |
| 目的探究心肌缺血模型大鼠的证候属性,对模型大鼠血瘀证相关信息进行客观评价。方法采用左冠状动脉结扎法复制大鼠心肌缺血模型,术后多个时间点,动态观察大鼠宏观体征,采集大鼠耳廓、足底及舌面图像,进行色度分析,同时在各时间点检测大鼠血液流变学指标;采用LARS法分析局部图像色度与血液流变学指标之间的相关性。结果模型组大鼠术后7～28天,局部图像色度和血液流变学指标与假手术组相比存在显著差异,术后7天时模型组大鼠足底R值(176.29±4.96)比假手术组(182.04±6.35)显著降低(P<0.05);术后14天时,模型组大鼠舌R值和G值显著降低(P<0.05);术后21天模型组大鼠耳廓G值(130.38±7.52)比假手术组(138.02±9.32)显著降低(P<0.05);术后28天,模型组大鼠舌B值(93.70±4.79)较假手术组(85.80±6.60)显著升高(P<0.01);模型组大鼠足底R、G、B值于术后14、21、28天均显著降低(P<0.05,P<0.01)。血液流变学指标中,模型组大鼠红细胞压积于术后14天和术后28天时与假手术组同时间点检测结果相比显著升高(均P<0.05),术后21天时模型组(45.38±1.96)比假手术组(41.50±2.12)显著升高(P<0.01);血小板聚集率,术后7天时模型组(49.35±6.74)比假手术组(37.83±9.94)显著升高(P<0.01),术后14天时模型组(58.19±7.57)比假手术组(48.00±10.44)亦显著升高(P<0.05);不同切变率下全血表观黏度和全血还原黏度也在模型组术后7～28天范围内存在不同程度改变。LARS回归显示局部图像色度与红细胞压积和血小板聚集率存在相关性。结论左冠状动脉结扎法造成的心肌缺血大鼠在术后7～28天内同时具备血瘀证和气虚证相关指征,诊断为气虚血瘀证。模型组大鼠局部图像色度的改变可能与其血中红细胞压积和血小板聚集率有关。 |
| 英文摘要: |
| Objective To investigate the syndrome property of myocardial ischemic(Ml) model rats, and to objectively evaluate the blood stasis pattern related information in them.Methods SD rats were established to Ml model by left anterior descending coronary artery(LAD) ligation.Global physical signs observation, colorimetric analysis on images of auricle,plantar and tongue,as well as the hemorrheologic indices detection were conducted dynamically at various time points after modeling.LARS regression was subjected to calculate the correlation between the color and hemorrheologic indices.Results Both colorimetric and hemorrheologic indices in Ml rats were significantly different to those in sham-operated rats(control) on day 7-28 after operation, showing significant difference between them in terms of plantar R on day 7(176.29±4.96 vs.182.04±6.35,P<0.05);R,G and B values of plantar on day 14,21 and 28;G and R values of tongue on day 14 and G value of auricle on day 21(130.38±7.52 vs.138.02±9.32,P<0.05),as well as B value of tongue on day 28 (93.70±4.79 vs.85.80±6.60,P<0.01 ).Results of hemorrheologic indices showed that hematocrit in Ml rats was higher than that in control on day 14 and 28(P<0.05),it was even higher on day 21(45.38±1.96 vs.41.50±2.12,P<0.01 );and platelet aggregation rate(PAR) was significantly higher in Ml on day 7 (49.35±6.74 vs.37.83±9.94,P<0.01 ) and day 14(58.19±7.57 vs.48.00±10.44,P<0.05).Besides, various degree of changes could also be seen in whole blood apparent viscosity and reduced viscosity at different shear rate on day 7-28 in the model group.LARS regression showed there existed correlation of local colorimetric indices with levels of hematocrit and PAR.Conclusion Ml model rat induced by LAD ligation can be diagnosed as the qi-deficiency and blood stasis syndrome,indications of the syndromes could be seen on 7-28 days after operation;the change of colorimetric indices of local images in Ml rats might be related with their levels of hematocrit and PAR. |
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