| 范永升,温成平,谢志军,孙静.3味解毒中药单用或不同组合对MRL/lpr小鼠分泌活性因子表达的影响[J].,2010,30(12):1306-1309 |
| 3味解毒中药单用或不同组合对MRL/lpr小鼠分泌活性因子表达的影响 |
| Effects of Three Chinese Herbal Antidotes (Herba Artemisiae annuae,Herba Hedyotis diffusae and Rhizoma Cimicifugae) and Their Different Combinations on Regulated on Activation Normal T Cell Expressed and Secreated Expression in MRL/lpr Mice |
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| DOI： |
| 中文关键词: 解毒祛瘀滋肾方 系统性红斑狼疮 MRL/lpr小鼠 分泌活性因子 |
| 英文关键词:Jiedu Quyu Ziyin Recipe systemic lupus erythematosus MRL/lpr mice regulated on activation normal T cell expressed and secreated |
| 基金项目:国家“十一五”科技支撑计划项目“重大疑难病(SLE)中医防治研究”(No.2006BAI04A10) |
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| 中文摘要: |
| 目的探讨解毒祛瘀滋肾方中3味解毒中药单用或不同组合对MRL/lpr小鼠血清及肾组织调节正常T细胞表达和分泌活性因子(regulated on activation normal T cell expressed and secreated,RANTES)表达的影响。方法 54只MRL/lpr小鼠随机分成9组,每组6只,分别以青蒿、蛇舌草、升麻、青蒿蛇舌草、青蒿升麻、蛇舌草升麻、青蒿蛇舌草升麻煎剂、泼尼松混悬液、生理盐水灌胃,12周后取血及肾脏,以ELISA法及RT-PCR法分别检测血清和肾组织中RANTES的表达。结果治疗12周末,泼尼松组的RANTES表达水平均显著下降;青蒿、升麻对RANTES表达水平的抑制作用不明显,蛇舌草、青蒿联合蛇舌草、青蒿联合升麻、蛇舌草联合升麻虽均有一定的抑制作用,但均不如青蒿联合蛇舌草及升麻的抑制作用明显。结论青蒿、蛇舌草、升麻三药联用能抑制RANTES的表达。 |
| 英文摘要: |
| Objective To explore the effects of three Chinese herbal antidotes,i.e.Herba Artemisiae annuae (A),Herba Hedyotis diffusae (H) and Rhizoma Cimicifugae (C),all were ingredients of Jiedu Quyu Ziyin Recipe,for adjusting the regulated on activation normal T cell expressed and secreated (RANTES),gene expression in serum and renal tissue of MRL/lpr mice.Methods Fifty-four MRL/lpr mice were randomized into 9 groups,with 6 in each,and intragastrically infused with A,H,C,A+H,H+C,A+C,A+H+C (all in dosage-form of decoction),prednisone suspension and physiological saline,respectively for 12 weeks.RANTES expression in serum and renal tissue of animals were detected with ELISA and RT-PCR at the end of the study.Results Levels of RANTES expression was significantly reduced in the prednisone treated group after treatment.Excepting no significant change being observed in the groups treated with A and C,the changes in the other groups were all milder than those in the group treated with A+H+C.Conclusion Chinese herbal antidotes A,H and C in combination can significantly inhibit the RANTES expression in serum and renal tissue of MRL/lpr mice. |
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